Protein Info for MPMX20_00051 in Enterobacter sp. TBS_079
Annotation: 6-phospho-beta-glucosidase GmuD
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 62% identical to GMUD_BACSU: 6-phospho-beta-glucosidase GmuD (gmuD) from Bacillus subtilis (strain 168)
KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 97% identity to enc:ECL_00048)Predicted SEED Role
"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)
MetaCyc Pathways
- cellulose degradation II (fungi) (2/3 steps found)
- neolinustatin bioactivation (2/3 steps found)
- linamarin degradation (1/2 steps found)
- lotaustralin degradation (1/2 steps found)
- linustatin bioactivation (2/4 steps found)
- coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
- α-tomatine degradation (1/6 steps found)
- firefly bioluminescence (2/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.21
Use Curated BLAST to search for 3.2.1.21
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (460 amino acids)
>MPMX20_00051 6-phospho-beta-glucosidase GmuD (Enterobacter sp. TBS_079) MKYAFPDNFWWGSASSALQTEGTREGETTWDYWFAREPNRFHNGVGPQHTSTFYQHWKTD IQLLKQLNHNSFRTSISWARLIPDGIGEVNPEAVDFYHQVIDELIEQGITPFITLFHFDM PMAMQEIGGWENRNVVDAYARYAQICFDLFGDRVLHWFTFNEPIVPVEGGYLYDFHYPNV VDFRRAATVAYHTVLAHAQAVRAYRAGHYVGEIGIVLNLTPSYPRSQNPADVKAAHIADL MFNRSFLDPVLRGEYPADLVALLKSYDQLPACKPEDSFLIAEGKIDLLGINYYQPRRVKC RDSAVNPQAPFMPEWFFDNYEMPGRKMNPYRGWEIYEPGIYDILINLRDNYGNPRCFISE NGMGVENEQRFIENGQINDQYRIDFISEHLAWLHKGISEGCQCLGYHMWTFIDNWSWCNA YKNRYGFIQLDLTTQQRTIKKSGEWFAATALNNSFDRESV