Protein Info for MMP_RS08725 in Methanococcus maripaludis S2

Annotation: Ni/Fe hydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 418 PF00374: NiFeSe_Hases" amino acids 39 to 117 (79 residues), 81.4 bits, see alignment E=3.8e-27 amino acids 230 to 407 (178 residues), 30.3 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 84% identical to VHUA_METVO: F420-non-reducing hydrogenase vhu subunit A (vhuA) from Methanococcus voltae

KEGG orthology group: K14126, F420-non-reducing hydrogenase subunit A [EC: 1.12.99.-] (inferred from 100% identity to mmp:MMP1694)

Predicted SEED Role

"CoB--CoM-reducing hydrogenase (Sec) alpha subunit @ selenocysteine-containing"

Isozymes

Compare fitness of predicted isozymes for: 1.12.99.-

Use Curated BLAST to search for 1.12.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LWL5 at UniProt or InterPro

Protein Sequence (418 amino acids)

>MMP_RS08725 Ni/Fe hydrogenase subunit alpha (Methanococcus maripaludis S2)
MGKVTIEPLSRLEGHGKVSITLDDAGKPTDVKLHITALRGFEQFVVGRPAEEVPRIVPRI
CGICQTPHHLASVKAVDAAWGAEVPSAAEKLRELMHLGNMMHSHALHFYYLAAPDFVLGP
DSDPAARNIIGVIGAAPEVAKKAIAMRRVGQSIVETIGGRAIHPVTGVPGGVSKALSEEK
RDELLNEIDEMIAYGQDGIALIKSLNEKYMDLAKTLGVIDTWYLGMVKDGKHNFYGDTLR
FMSPDGKQTTDFKPSEYLDNIGEHVVGHNYVKYPYNKKVGYPDGLYRVGPLAMLNVCDSM
PTPLAEEARKDFADTFGKPANQSLAYNHARLIELLASCERVKELLEDSEITSTDIKAEVE
PKAGNGVGAIYAPRGTLFHNYETDDKGIVVKANMIVASTHNVPTMEKAIQQAAEVVFK