Protein Info for MMP_RS08690 in Methanococcus maripaludis S2

Annotation: RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 TIGR00425: putative rRNA pseudouridine synthase" amino acids 2 to 318 (317 residues), 487.2 bits, see alignment E=2.1e-150 PF08068: DKCLD" amino acids 2 to 46 (45 residues), 58.2 bits, see alignment 1.7e-19 PF01509: TruB_N" amino acids 51 to 163 (113 residues), 79.6 bits, see alignment E=8.1e-26 PF21238: Pus10_C" amino acids 92 to 169 (78 residues), 27.5 bits, see alignment E=5.7e-10 PF16198: TruB_C_2" amino acids 165 to 230 (66 residues), 87.1 bits, see alignment E=1.8e-28 TIGR00451: uncharacterized domain 2" amino acids 231 to 304 (74 residues), 31.7 bits, see alignment E=1.5e-11 PF01472: PUA" amino acids 234 to 307 (74 residues), 64.2 bits, see alignment E=2.1e-21

Best Hits

Swiss-Prot: 100% identical to TRUB_METMP: Probable tRNA pseudouridine synthase B (truB) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K03177, tRNA pseudouridine synthase B [EC: 5.4.99.12] (inferred from 100% identity to mmp:MMP1686)

Predicted SEED Role

"tRNA pseudouridine 55 synthase (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LWM3 at UniProt or InterPro

Protein Sequence (333 amino acids)

>MMP_RS08690 RNA-guided pseudouridylation complex pseudouridine synthase subunit Cbf5 (Methanococcus maripaludis S2)
LELIVKEESKTDYNYGSDPYNRDIKTLLNTGLVVIDKPSGPTSHEVAAWVRNMLNLVKAG
HGGTLDPKVTGALPVALGNTTKCVPIWHIPPKEYVCLMHLHDDAELVDIENIFKEFTGRI
HQRPPLKAAVKRSLRIRKIYEIEILEIDGRDILFRTKCQSGTYLRKLVDDMGEALGTSAH
MQELRRTISGPFYENEAVYLQDLLDAYIFWKEDGNEEELRKIVKPLEYGLQHLKKIIIKD
SAVDAVCHGATLYSSGISKIEKGLGTDEVVLIETLKGEAVAVGKPLMNTKDMLKTEEGEV
VEITRVIMEPGIYPRIWKKRNKNDKSKPELKKK