Protein Info for MMP_RS08255 in Methanococcus maripaludis S2

Annotation: bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 TIGR00521: phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase" amino acids 15 to 393 (379 residues), 376.4 bits, see alignment E=8.6e-117 PF02441: Flavoprotein" amino acids 18 to 177 (160 residues), 90.6 bits, see alignment E=8.3e-30 PF04127: DFP" amino acids 189 to 370 (182 residues), 163.4 bits, see alignment E=5.6e-52

Best Hits

Swiss-Prot: 60% identical to COABC_METJA: Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K13038, phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase [EC: 4.1.1.36 6.3.2.5] (inferred from 100% identity to mmp:MMP1606)

MetaCyc: 60% identical to CoaBC monomer (Methanocaldococcus jannaschii)
Phosphopantothenate--cysteine ligase. [EC: 6.3.2.5]; Phosphopantothenoylcysteine decarboxylase. [EC: 6.3.2.5, 4.1.1.36]

Predicted SEED Role

"Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)" in subsystem Coenzyme A Biosynthesis (EC 4.1.1.36, EC 6.3.2.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.36, 6.3.2.5

Use Curated BLAST to search for 4.1.1.36 or 6.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LWU8 at UniProt or InterPro

Protein Sequence (396 amino acids)

>MMP_RS08255 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC (Methanococcus maripaludis S2)
MHPTKTLHGAKSKLLDGKKLVVAVTSSIAAIEAPRLMRELIRHGAEVYCIMTEETKDIVG
KYSLEFGCGNEVMDKITGKIEHVDLYNKCDGMIVFPATANIISKISLNIADNIVNTTAMM
FLEKKPIIIAPSMHQNMLDTVRDHIKNLSEKNNVFVMGSKMEEEKAKAISIDEVSKLAID
VFSDRKEERKKVLILSGGTAEPIDKVRVITNLSSGKTGVSLAEEFCRQNYDVDVIKGLGK
DVPYYINSENVITSQEMLKKALELGESADIIISCAAISDYAPETNFEGKLSSDIKTQVIK
LKQTPKVLEELRKKFPKKIIVGYKAEYGINLDELKEKAISRLEKYNLDVIIANDLSKHYF
GDDLNEVLIIDKEKALKVSGSKDEISKKIVEIVNNL