Protein Info for MMP_RS08005 in Methanococcus maripaludis S2

Annotation: coenzyme-B sulfoethylthiotransferase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 TIGR03257: methyl-coenzyme M reductase, beta subunit" amino acids 5 to 435 (431 residues), 786.1 bits, see alignment E=4.7e-241 PF02783: MCR_beta_N" amino acids 6 to 187 (182 residues), 266.4 bits, see alignment E=1.1e-83 PF02241: MCR_beta" amino acids 189 to 436 (248 residues), 455 bits, see alignment E=7.5e-141

Best Hits

Swiss-Prot: 91% identical to MCRB_METVA: Methyl-coenzyme M reductase subunit beta (mcrB) from Methanococcus vannielii

KEGG orthology group: K00401, methyl-coenzyme M reductase beta subunit [EC: 2.8.4.1] (inferred from 100% identity to mmp:MMP1555)

MetaCyc: 63% identical to beta subunit (Methanothermobacter thermautotrophicus)
Coenzyme-B sulfoethylthiotransferase. [EC: 2.8.4.1]

Predicted SEED Role

"Methyl coenzyme M reductase beta subunit (EC 2.8.4.1)" in subsystem Methanogenesis (EC 2.8.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.4.1

Use Curated BLAST to search for 2.8.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LWZ9 at UniProt or InterPro

Protein Sequence (443 amino acids)

>MMP_RS08005 coenzyme-B sulfoethylthiotransferase subunit beta (Methanococcus maripaludis S2)
MVKYEDKISLYDAKGNLVAENVPLEAISPLYNPTIKSMVKNIKRTVAVNLAGIEGTLAAG
KIGGKGCQVPGRTLDISAVSNAQAIADEVEKILKVSEDDDTAVKIINGGKQLAVQVPTAR
LDVAAEYSVSMLSTAMALKEALIKTFNIDMFDGSTVHAAIVGNYPQVMDYAGGNIASLLG
APSMMEGLGYALRNIPVNHAVATTKKNMMNAIAFSSVMEQTATFEMGDAVGSFERQHLLG
LAYQGLNADNLVIDFIKANAKGTVGSVVETVIDRAIADGVIVVDKTMSSGFNMYKPADVN
KWNAYAAAGLVAAVAVSCGAARAAQNVASVILYFNDILEYETGLPGVDYGRSMGTAVGFS
FFSHSIYGGGGPGIFNGNHVVTRHSKGFAIPPVCAAMCADAGTQMFSPEHTSGLVGSVYS
AFDEFREPMKYVIEGALSIKDQF