Protein Info for MMP_RS07760 in Methanococcus maripaludis S2

Annotation: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 TIGR00132: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit" amino acids 4 to 430 (427 residues), 547.9 bits, see alignment E=9.5e-169 PF01425: Amidase" amino acids 6 to 421 (416 residues), 417.2 bits, see alignment E=4.9e-129

Best Hits

Swiss-Prot: 100% identical to GATA_METMP: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 100% identity to mmp:MMP1510)

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LX43 at UniProt or InterPro

Protein Sequence (431 amino acids)

>MMP_RS07760 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA (Methanococcus maripaludis S2)
MITDRVSDYLEKIEKSDVNAFIDVNGEKVLKEAEELEKNDTLKNKPLYGKIVAVKSNINV
KGYKISCASKTLEKYVGTYDATVVKKLRSQGALIVGMTNMDEFAGGSSGETSCYGPTKNP
AAMDRIPGGSSSGSAAAVAADLCDMAIGSDTGGSIRNPASHCGIVGFKPSYGVVSRQGLC
DLAMSFDQIGPLTKNAEDALVLTNAIKGIDRSDSTSLETPKFEKKDISNYKIGVVKEFMD
VTDEKIRNEIEKGIEVFKDMGCKIVDLSYKYIDLALPTYYLINYVEFFSATRKYDGRRYG
EFIEEACGEEVLRRILIGKHISEQEFSGKYYKKALQARKSMKKEMLGLFNSADLIVGPTV
PKLPHKLGEDVSPMEMYAYDVLTVPTNICGICSGVVRCGNISGVPVGLQIQGAPLEDEKV
LSAMIEFEKNY