Protein Info for MMP_RS07035 in Methanococcus maripaludis S2

Annotation: DNA-directed RNA polymerase subunit A''

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 386 TIGR02389: DNA-directed RNA polymerase, subunit A''" amino acids 14 to 376 (363 residues), 558.7 bits, see alignment E=2.9e-172 PF04998: RNA_pol_Rpb1_5" amino acids 51 to 326 (276 residues), 187.9 bits, see alignment E=1.3e-59

Best Hits

Swiss-Prot: 100% identical to RPOA2_METMP: DNA-directed RNA polymerase subunit A'' (rpoA2) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K03042, DNA-directed RNA polymerase subunit A'' [EC: 2.7.7.6] (inferred from 100% identity to mmp:MMP1364)

Predicted SEED Role

"DNA-directed RNA polymerase subunit A'' (EC 2.7.7.6)" in subsystem RNA polymerase archaeal (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LXI7 at UniProt or InterPro

Protein Sequence (386 amino acids)

>MMP_RS07035 DNA-directed RNA polymerase subunit A'' (Methanococcus maripaludis S2)
MQMADLEKKLENSVLPPLLKRELSEKILREEISEEYLVDEIISETTRAYERTLVEPGEAV
GVVAAQSIGEPGTQMTMRTFHYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTIYLNDEF
KGNREKAATVAKNIESTNVESVSEDISVDLVNECITIILNNQQLESRGLTVPDVIDAIKS
KMKLKIEDHENVLNLKIKTPSLKALRKRLPKVRAIHLKGVQNIKRVIIRKEVDEYILYSE
GSNIKEVFDIEGVDTTKTTTNNIVEIQDVLGIEAARNAIIYEMDATLGNQGLTVDKRHLM
MVADLMCTDGVVKPIGRHGIGGEKASVLARAAFEETVKHLYSASMRGYVDELGGVVENII
VGKPISMGTGCIDICIDKSYEEGKEL