Protein Info for MMP_RS06675 in Methanococcus maripaludis S2

Annotation: ADP-specific phosphofructokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 PF04587: ADP_PFK_GK" amino acids 14 to 451 (438 residues), 381.2 bits, see alignment E=3e-118 TIGR02045: ADP-specific phosphofructokinase" amino acids 14 to 459 (446 residues), 601.2 bits, see alignment E=5.4e-185

Best Hits

Swiss-Prot: 58% identical to K6PF_METJA: Bifunctional ADP-specific glucokinase/phosphofructokinase (pfkC) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K00918, ADP-dependent phosphofructokinase/glucokinase [EC: 2.7.1.146 2.7.1.147] (inferred from 100% identity to mmp:MMP1296)

MetaCyc: 49% identical to ADP-dependent phosphofructokinase subunit (Pyrococcus furiosus)
ADP-specific phosphofructokinase. [EC: 2.7.1.146]

Predicted SEED Role

"ADP-dependent glucokinase (EC 2.7.1.147) @ ADP-dependent phosphofructokinase (EC 2.7.1.146)" (EC 2.7.1.146, EC 2.7.1.147)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.146 or 2.7.1.147

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LXQ3 at UniProt or InterPro

Protein Sequence (463 amino acids)

>MMP_RS06675 ADP-specific phosphofructokinase (Methanococcus maripaludis S2)
MEIINHFKDFSNVSIFLAYNVNVDALKYLTDLSDIEELKENFSDSEIKTKIEEYPRTIEK
PIDFVARLIHAMKSGKPAEVPLKNNLEIDTFLNRLTYNEERIGGQVGIISNLLSILNLKK
IIFYSPILAKKQAEMFENNENLVFPNITNGKLVLKKPIESFKNDELKINRIFEYKEDIEF
YLENEKITTPQSNRFIVASRPENLRIEIKDELKSHLPEIGQLVDCAIISGVQAIKEEYSD
GKTSEYYLNKVKEDIKSLKKENKDLKVHFEFASIQNTEMRKKIAESILPEVDCVGMDETE
IANIIHVLGYEELSEGILKHSKIEDVLKASKILLEKYNLEGMQVHTMYYIMYLCKKGGIL
SDESLEKTLEFATVLASTKAALGQISSIEDLKTGLKIPHNKHGELLKEIVENISKEKELG
GYKIILVPSRIVENPKSTVGLGDTISAGAFVGYVSELKKLKNK