Protein Info for MMP_RS06505 in Methanococcus maripaludis S2

Annotation: Glu-tRNA(Gln) amidotransferase subunit GatE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00134: glutamyl-tRNA(Gln) amidotransferase, subunit E
  • amino acids 4 to 629 (626 residues), 962 bits, E=5.7e-294
• PF02934: GatB_N
  • amino acids 11 to 444 (434 residues), 209.7 bits, E=1e-65
• PF02938: GAD
  • amino acids 296 to 389 (94 residues), 81.9 bits, E=6.6e-27
• PF02637: GatB_Yqey
  • amino acids 483 to 626 (144 residues), 106.3 bits, E=2.7e-34
• PF09424: YqeY
  • amino acids 578 to 626 (49 residues), 30.2 bits, 9.3e-11

Best Hits

Swiss-Prot: 100% identical to GATE_METMP: Glutamyl-tRNA(Gln) amidotransferase subunit E (gatE) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K03330, glutamyl-tRNA(Gln) amidotransferase subunit E [EC: 6.3.5.7] (inferred from 100% identity to mmp:MMP1265)

MetaCyc: 52% identical to glutamyl-tRNA(Gln) amidotransferase subunit E (Methanothermobacter thermautotrophicus Delta H)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]

Predicted SEED Role

"Glutamyl-tRNA(Gln) amidotransferase transferase subunit (EC 6.3.5.7)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.3.5.7)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (4/8 steps found)
• L-glutamate and L-glutamine biosynthesis (3/7 steps found)
• superpathway of L-citrulline metabolism (6/12 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Glutamate metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.7

Use Curated BLAST to search for 6.3.5.7

Sequence Analysis Tools

See Q6LXT2 at UniProt or InterPro

Protein Sequence (631 amino acids)

>MMP_RS06505 Glu-tRNA(Gln) amidotransferase subunit GatE (Methanococcus maripaludis S2)
MDYDYEKLGLKVGLEIHQQLNTKRKLFCNCPTKIRDDEPHGEIERVLRPSQSEMGHVDKA
ALLESKKEKKFIYQYYNDTTCLVELDDEPPHDVAPEAVDTALEVSTLMNMKMADEVQIMR
KMVIDGSNTSGFQRTMFVSQEGFIETEYGNIGVTSLCLEEDACKKIEDGKDYTKYCVDRL
GIPLLEITTEPDITSPKMGKEAARRIGTILRATGKVKRGLGTIRQDVNISIKNGARIEVK
GVQNLDLIEKIIENEVTRQISLNEIKEELLKRNAEVVDEIKDITELLKDTESKVLKNALK
NKGVIRAILLKGFSGMIGREVQPGRRLGTEFSDRGKVLGGVGGLFHTDELPKYGITEEEV
IKLKEYMNCGENDAVILVADKKNKVERALNAVIERAKESMIGIPEETRKALDDGNTSYLR
PLPGAARMYPETDVPTITITEEKLEFVRNNLPEMPEEKLVRFVKEYELNEDLAKQMVMSY
HVDLFETLSKKYSKIKPTLIATTLEATIKEIKREGLDTDLLTEEHLEELFNGLSEDKMSK
EAVPDVIKGFIENPTKKLDEILEIKGMSSMSVEEVESIIEDIINQNISQVNEKGMGAMGL
LMGRCMAQLRGKADGKLINTTLQKKLKEKVQ