Protein Info for MMP_RS06280 in Methanococcus maripaludis S2

Annotation: alpha-glucan family phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 TIGR02094: alpha-glucan phosphorylases" amino acids 5 to 288 (284 residues), 210 bits, see alignment E=3.8e-66 PF00343: Phosphorylase" amino acids 291 to 499 (209 residues), 46.8 bits, see alignment E=7.7e-17

Best Hits

Swiss-Prot: 73% identical to Y1631_METJA: Uncharacterized protein MJ1631 (MJ1631) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to mmp:MMP1220)

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LXX5 at UniProt or InterPro

Protein Sequence (520 amino acids)

>MMP_RS06280 alpha-glucan family phosphorylase (Methanococcus maripaludis S2)
MKNTAYFSMEFAIDQSLKTYAGGLGFLAGSHFRAAKRLNVPLVGVSMLWSYGYYDQVRDR
EGRMKVENIRKYYDFLTDIDLKVPVVINGTNIWVKAYKLEENTFNTCPIYFLTTDIPEND
HLSRTISYGLYDGNNLTHIAQEIVLGIGGYKVIRECENVKRYHINEAHSLPVAFKMLEDY
GTDYVKEHLLFTTHTPIPAGNETQDINLLKSMGFFGSIDTKTAEKYGGNPFNLTVAALRM
CKRSNAVSELHRDTSEKMWAWVTNREPIMNITNAQDKYYWQDRQIREAAGKKDIKMLRER
KMELKKQLFEEVADQTGSLFDPNILTVVWARRFADYKRPSFLLRDEKRFKNLLKTGKIQV
IWAGKPHPADSNAQITFNWIVSKTRELKNVSVLTGYELKLSKLLKQGSDVWLNTPRRPNE
ASGTSGMTASMNASIHMSTMDGWHVEWAKAYPKDSFTIGDGVNLDEDYEANEMYKHLEKT
IKIYDTDEWWEKAINSVNHVVEYFDAERMIMEYTKKFYSD