Protein Info for MMP_RS05950 in Methanococcus maripaludis S2

Annotation: nickel-dependent hydrogenase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 PF00374: NiFeSe_Hases" amino acids 42 to 113 (72 residues), 43.4 bits, see alignment E=2.5e-15 PF00346: Complex1_49kDa" amino acids 118 to 365 (248 residues), 149.4 bits, see alignment E=1.1e-47

Best Hits

Swiss-Prot: 73% identical to Y1027_METJA: Uncharacterized protein MJ1027 (MJ1027) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K14123, energy-converting hydrogenase B subunit N (inferred from 100% identity to mmp:MMP1153)

Predicted SEED Role

"Energy conserving hydrogenase Ehb large subunit (protein N)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LY40 at UniProt or InterPro

Protein Sequence (375 amino acids)

>MMP_RS05950 nickel-dependent hydrogenase large subunit (Methanococcus maripaludis S2)
MYEGEIAIGPVHSTMLEPHRLRLFIEDEIVKDAELTIGVNHRGVERLMEGLPVEKACILC
EKICGICSHIHLWSAARLVEIGCNIEIPERANHIRIIVEELERLHSHTLLFGHAFEILGH
ETMSMRCFMLREPIMQVFFEISGSRVHYSCPIIGGIRPRCNISETQIPHILDKISKYEEG
LNKFLERMLNDPMIISRVKDVGVMDRKTAAKFHAVGPTARGSNVKSDMRKWGWVPEYDPY
DFDEILFDSGDVFARLAVRFYECLESVKIIRQALDALKDTSDKRIYNPNYELHEFKPINC
YTEAQRGEQYYSYGLDDEGLVRQAKIRTPTATNLGAMEDIVKGYHVSDAELIIASCDPCF
TCTDRLMVLKEPFKK