Protein Info for MMP_RS05930 in Methanococcus maripaludis S2
Annotation: 3-isopropylmalate dehydratase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 77% identical to LEUC_METJA: Isopropylmalate/citramalate isomerase large subunit (leuC) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 100% identity to mmp:MMP1149)MetaCyc: 77% identical to isopropylmalate isomerase large subunit (Methanocaldococcus jannaschii)
(R)-2-methylmalate dehydratase. [EC: 4.2.1.35]; 3-isopropylmalate dehydratase. [EC: 4.2.1.35, 4.2.1.33]; 4.2.1.33 [EC: 4.2.1.35, 4.2.1.33]; RXN-7744 [EC: 4.2.1.35, 4.2.1.33]
Predicted SEED Role
"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (16/17 steps found)
- L-isoleucine biosynthesis II (8/8 steps found)
- L-leucine biosynthesis (6/6 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
- 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- C5-Branched dibasic acid metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35
Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (archaea)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q6LY44 at UniProt or InterPro
Protein Sequence (424 amino acids)
>MMP_RS05930 3-isopropylmalate dehydratase large subunit (Methanococcus maripaludis S2) MAMTLAEKILAKASGNSEVSPGDIVMAKVETAMVHDITGPLSVNTLKKEGISKVWDNEKI VIPFDHQIPADSINAAENHILMRNFVKEQNIKHFYDIREGVCHQVLPEKGHIVPGTVVVG ADSHTCTYGAFGAFATGIGSTDMAAAFATGELWFKVPETLYFNITGELKPEVMSKDVILS IIGMVGADGATYKAAQFAGNTVDNMTIASRMTMSNMAIEMGGKAGLIAPDDKTINYVKNA MEANNTAKPFELVLGDKNAEFEEKFEIDVSNLEPVMACPHNVDNVKAVREVAGTPIDQVF IGSCTNGRLEDLRAALNVIEKHGGISKDIRVVVTPASRSIMLEAIDEGLIKKFYQYGCVV TNPSCSACMGALYGLLGPGEVGIATSNRNFRGREGSLESDVYLASPITAAACAVKGEIVD PRDL