Protein Info for MMP_RS05600 in Methanococcus maripaludis S2

Annotation: AglZ/HisF2 family acetamidino modification protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03572: glycosyl amidation-associated protein WbuZ
  • amino acids 1 to 229 (229 residues), 310.7 bits, E=2.8e-97
• PF00977: His_biosynth
  • amino acids 5 to 230 (226 residues), 193.3 bits, E=4.5e-61
• PF03060: NMO
  • amino acids 154 to 228 (75 residues), 29.8 bits, E=4.3e-11

Best Hits

Swiss-Prot: 49% identical to HIS62_PSEAE: Putative imidazole glycerol phosphate synthase subunit hisF2 (hisF2) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02500, cyclase [EC: 4.1.3.-] (inferred from 100% identity to mmp:MMP1083)

MetaCyc: 32% identical to imidazole glycerol phosphate synthase subunit HisF (Escherichia coli K-12 substr. MG1655)
GLUTAMIDOTRANS-RXN [EC: 4.3.2.10]

Predicted SEED Role

"Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)" in subsystem Histidine Biosynthesis (EC 4.1.3.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (35/46 steps found)
• L-histidine biosynthesis (9/10 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (4/8 steps found)
• L-glutamate and L-glutamine biosynthesis (3/7 steps found)
• superpathway of L-citrulline metabolism (6/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.-

Use Curated BLAST to search for 4.1.3.- or 4.3.2.10

Sequence Analysis Tools

See Q6LYA8 at UniProt or InterPro

Protein Sequence (244 amino acids)

>MMP_RS05600 AglZ/HisF2 family acetamidino modification protein (Methanococcus maripaludis S2)
MYRNRIIPCLLLKDERLVKTVKFKDPNYIGDPLNAVRIFNDKEADELFFIDISASKRESI
NFDLLKKISSQSFMPLGYAGGIKCLNDAKKIFSIGFEKISLNTIVLKNPDLITEISKIYG
SQSVLVTIDVKKNFFGKYYVYNHLKKKITKYNPVDFARKMEVLGAGELVINCVDNDGVMK
GYNLKLIGEISKAVKIPIVALGGAGSLKDLDDAINVGASAAGAGSLFIYYGPHKAVLINY
PRGD