Protein Info for MMP_RS05500 in Methanococcus maripaludis S2
Annotation: 2-isopropylmalate synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 71% identical to LEU1_METJA: 2-isopropylmalate synthase (leuA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 100% identity to mmp:MMP1063)MetaCyc: 50% identical to (R)-citratemalate synthase subunit (Methanocaldococcus jannaschii)
RXN-7743 [EC: 2.3.3.21]
Predicted SEED Role
"(R)-citramalate synthase (EC 2.3.1.182); 2-isopropylmalate synthase (EC 2.3.3.13)" (EC 2.3.1.182, EC 2.3.3.13)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (16/17 steps found)
- L-isoleucine biosynthesis II (8/8 steps found)
- L-leucine biosynthesis (6/6 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Pyruvate metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.182
Use Curated BLAST to search for 2.3.1.182 or 2.3.3.13 or 2.3.3.21
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (archaea)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q6LYC8 at UniProt or InterPro
Protein Sequence (514 amino acids)
>MMP_RS05500 2-isopropylmalate synthase (Methanococcus maripaludis S2) MESYLNSNEIIKNSLKSMKLPEKVRVFDTTLRDGEQTPGVSLMPDQKMDIAKNLSEIGVD AIEAGFPVSSEGEQESIKNITSLGLDAEICGLARAVKKDIDVAIDCGVDSIHTFIATSPL HREYKLKMSKEKIIDIAVESIEYIKEHGVIVEFSAEDATRTELDYLKEVYKKAVEAGADR INVPDTVGVMVPNSMSYLIKELKNDIKVPISVHCHNDFGIAVSNSVAAVEAGAEQVHCTV NGLGERAGNASLEETVMTLNMVYGINTNVNTKMLTKLSKLVSHYTGIKTQPNKAIVGENS FAHESGIHAHGVLANALTYEPIDPEIVGNRRRIVLGKHSGAHAIKSKLSEIGVEVGEIVS EEQFCEIVERVKAIGDKGKIVTDADVMAIAEDITKRTLKSERIVDLEQFAVITGNHVLPT ASVALKVKDKIYKTSELGVGPVDAALKAIQAAVGENIRLNEYNISAISGGTDAIAEVTVR LENQEKEVIAKATGDDVVKASVEAVIDGINKLMG