Protein Info for MMP_RS05265 in Methanococcus maripaludis S2

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 492 TIGR02090: isopropylmalate/citramalate/homocitrate synthases" amino acids 2 to 364 (363 residues), 567.7 bits, see alignment E=5.4e-175 PF00682: HMGL-like" amino acids 2 to 259 (258 residues), 267.9 bits, see alignment E=1e-83 PF08502: LeuA_dimer" amino acids 355 to 491 (137 residues), 98.4 bits, see alignment E=3.1e-32

Best Hits

Swiss-Prot: 70% identical to CIMA_METJA: (R)-citramalate synthase CimA (cimA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K09011, D-citramalate synthase [EC: 2.3.1.182] (inferred from 100% identity to mmp:MMP1018)

MetaCyc: 70% identical to (R)-citratemalate synthase subunit (Methanocaldococcus jannaschii)
RXN-7743 [EC: 2.3.3.21]

Predicted SEED Role

"(R)-citramalate synthase (EC 2.3.1.182)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 2.3.1.182)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.182

Use Curated BLAST to search for 2.3.1.182 or 2.3.3.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LYH3 at UniProt or InterPro

Protein Sequence (492 amino acids)

>MMP_RS05265 2-isopropylmalate synthase (Methanococcus maripaludis S2)
MVNIFDTTLRDGEQTPGVSLSPSEKLELAIKLDELGVNIIEAGSAITSKGEREAMKLVTG
QNLNAEISSFVRALPVDIDAALSCDVDSVHLVVPTSDIHMTYKLRKSREENLKDAMHAVE
YAKDHGLIVELSAEDATRSDVEFLKELFLKGEELKADRICVCDTVGILTPLKAIDLIKTM
KNTIKIPVSIHCHNDFGMATANTLSAISAGADQCHVTINGIGERAGNASLEEVVMSLKSL
YDIETGIDTEKLHKISRIVSKYMKIPVPANKALVGDNAFAHEAGIHVDGLMKSTETYEPI
HPETVGNRRKIILGKHSGKAALKYKLEIMNIGLSHEEFEETYSKIKAFGDLGKYISDVDL
KTIINQVRGVELERKVILDEITVVSGNKVSPLASINLKFANDCNIKDNVREAAYGLGPVD
AAINAVKKALTGVADIELEDYSVRAMTGGTDALIEVVVHLRKGNEVVEVKKAHGDVVMAS
VEAMMDGINLLI