Protein Info for MMP_RS05100 in Methanococcus maripaludis S2

Annotation: CO dehydrogenase/acetyl-CoA synthase complex subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00314: CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit
  • amino acids 10 to 771 (762 residues), 1276.5 bits, E=0
• PF03063: Prismane
  • amino acids 74 to 129 (56 residues), 35.5 bits, 7.6e-13
  • amino acids 161 to 655 (495 residues), 177.3 bits, E=7.7e-56
• PF13237: Fer4_10
  • amino acids 405 to 458 (54 residues), 25.9 bits, 1.2e-09
• PF13187: Fer4_9
  • amino acids 407 to 461 (55 residues), 28.5 bits, 1.9e-10

Best Hits

Swiss-Prot: 71% identical to ACDA_METTH: Acetyl-CoA decarbonylase/synthase complex subunit alpha (cdhA) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

KEGG orthology group: K00192, acetyl-CoA decarbonylase/synthase complex subunit alpha [EC: 1.2.99.2] (inferred from 100% identity to mmp:MMP0985)

MetaCyc: 45% identical to acetyl-CoA decarbonylase/synthase complex alpha subunit (Methanosarcina thermophila)
2.3.3.M4 [EC: 2.3.3.M4]; 1.2.7.4,2.3.3.M4,2.3.3.M4,2.3.3.M5 [EC: 2.3.3.M4, 1.2.7.4, 2.3.3.M5]

Predicted SEED Role

"CO dehydrogenase/acetyl-CoA synthase subunit alpha, CO dehydrogenase subcomplex (EC 1.2.99.2)" in subsystem Carbon monoxide induced hydrogenase (EC 1.2.99.2)

MetaCyc Pathways

• gluconeogenesis II (Methanobacterium thermoautotrophicum) (16/18 steps found)
• reductive acetyl coenzyme A pathway II (autotrophic methanogens) (5/6 steps found)
• carbon tetrachloride degradation II (4/6 steps found)
• methanogenesis from acetate (4/6 steps found)
• hydrogen production VI (1/2 steps found)
• methoxylated aromatic compound degradation II (6/9 steps found)
• superpathway of methanogenesis (11/21 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (35/56 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Trinitrotoluene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.2

Use Curated BLAST to search for 1.2.7.4 or 1.2.99.2 or 2.3.3.M4 or 2.3.3.M5

Sequence Analysis Tools

See Q6LYK6 at UniProt or InterPro

Protein Sequence (780 amino acids)

>MMP_RS05100 CO dehydrogenase/acetyl-CoA synthase complex subunit alpha (Methanococcus maripaludis S2)
MVMAYKKMDKKLDDDFWKTKNLSLSIGEVSVGKPKQELDEPMGPTPKPSVTDLRSWDFKL
LKRYPPFYAPFCDMCCLCTYGKCDLLGKKGACGIDAEAQQARTVLLASCIGTAAHAGHGR
HMLHHVIKEKGKDFPIDMGKNIDIEAPIIRTIIGKKPVTVSDLDESLSYVEEQLSHLLSA
CHTGQEGNSLDFESKALHAGMMDDLAREVADITQIVAYDMPRGDGDEPLVELGLGTVDKD
KPVVLCIGHNVAPGAEILDYVEDNDLYEDVEICGICCTAIDITRYNQAAKVIGPLSKQMK
FIRSGVSDVIVVDEQCVRTDVLEEAKRNGAVVIATTDKMCLGLPDMTKEDPELIINELVN
GNIDGALILEPKKVGKVAVEVAKKIAKRRESLKCLPDISEIPEIAKECTECGWCVRVCPN
NRPIMDAVTSAAKGDLTKLANLYEHDMCYTCGRCEEECERNLPLVSFITKAGDHFVKNQK
FKMRAGRGPVQDVEIRKVGGPIVLGDIPGVVAFVGCSNFPNGGIEIAKMAEELLKRRYIV
VATGCSAMSIGEYIDEDGLTLYEKYEGIFDASGLANIGSCVSNAHISGACIKIANIFAKK
PLSGNFEEVADYILNRVGACGVAWGAFSQKAAAIAAGVNRWGIPAVLGPHSSKYRRLYLG
RIDKEEMWDLNDLRTGETVKGEPAPEHLLYAAETMEEALVSTVKMCIRPNDTPKGRQLKL
SNYIDLYKKYFGELPPDLELFVRNERDIPITHKKEVMDDLTSKNWTPREAPKEPSLLERK