Protein Info for MMP_RS05030 in Methanococcus maripaludis S2

Annotation: adenylosuccinate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00928: adenylosuccinate lyase
  • amino acids 6 to 441 (436 residues), 561.9 bits, E=4.7e-173
• PF00206: Lyase_1
  • amino acids 12 to 295 (284 residues), 188.3 bits, E=2.4e-59
• PF10397: ADSL_C
  • amino acids 363 to 441 (79 residues), 88.2 bits, E=3.7e-29

Best Hits

Swiss-Prot: 73% identical to PUR8_METJA: Adenylosuccinate lyase (purB) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 100% identity to mmp:MMP0971)

MetaCyc: 73% identical to adenylosuccinate lyase (Methanocaldococcus jannaschii)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]; 4.3.2.- [EC: 4.3.2.2]

Predicted SEED Role

"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (35/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (17/21 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)
• inosine-5'-phosphate biosynthesis II (4/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (4/5 steps found)
• superpathway of purine nucleotide salvage (10/14 steps found)
• inosine-5'-phosphate biosynthesis I (4/6 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (4/7 steps found)
• dZTP biosynthesis (2/5 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (17/26 steps found)
• tetrahydromethanopterin biosynthesis (6/14 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.2

Sequence Analysis Tools

See Q6LYM0 at UniProt or InterPro

Protein Sequence (449 amino acids)

>MMP_RS05030 adenylosuccinate lyase (Methanococcus maripaludis S2)
MAIHPIDFRYGTPEMKKVWGEETKLQKMLEVEAALALAESELGMIPKEAAEEINRKKSTE
FVKLERVKEIEMATRHDVVSVVKAFAEQCEGTAGEYIHFGSTSNDIIDTAQSLQFKDAFE
VLESKLKILRGELLKKAEEHKNTVCIGRTHGQHAVPTTYGMKFALWATEIQRHIERLESS
KKRLCVSMITGAVGTMAAIGENGILVHKRVGEILSLDPVLISNQVVQRDRHGEFMATLAL
IAQTLNKIGVTVRSMQRSEIKELEEEFDASKQTGSSTMPHKRNPITFEQICGLSRIVKAN
ALAEFDNIPLWEERDLTNSSSERCLFPESCVLLDHIINLSIKGMRKLSVNLENVNKNLEM
TKGLIMAERVMMFLANSGMGRQTGHETVRKCAMKAHDEGRHLKEVLLEEPEVMKYVSLDE
IEKMFDYKTYIGLAPEIVENVLNEAKNWE