Protein Info for MMP_RS04955 in Methanococcus maripaludis S2

Annotation: DNA topoisomerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 823 PF01751: Toprim" amino acids 4 to 136 (133 residues), 59.1 bits, see alignment E=5.9e-20 TIGR01057: DNA topoisomerase I" amino acids 6 to 622 (617 residues), 850.4 bits, see alignment E=3.4e-260 PF01131: Topoisom_bac" amino acids 152 to 555 (404 residues), 437.9 bits, see alignment E=6.1e-135 PF01396: zf-C4_Topoisom" amino acids 596 to 630 (35 residues), 40.1 bits, see alignment (E = 3.8e-14) amino acids 682 to 717 (36 residues), 49.9 bits, see alignment (E = 3.4e-17) amino acids 726 to 764 (39 residues), 61.8 bits, see alignment 6.3e-21

Best Hits

Swiss-Prot: 63% identical to TOP1_METJA: DNA topoisomerase 1 (topA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 100% identity to mmp:MMP0956)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LYN4 at UniProt or InterPro

Protein Sequence (823 amino acids)

>MMP_RS04955 DNA topoisomerase I (Methanococcus maripaludis S2)
MTGLIICEKPNVAKKIAEALGKPKKKSHNSVPYYELERNGESIVVASAVGHLYTLQEKTK
TKFGDYPVYDINWVPASTLDEKKYIQKYIDALKKVSKDANKFYVATDWDIEGELIGYHAL
NFSCGQKNAHRLRFSSLTKKEILKAYENPNEIDFGLVDAGDSRHKIDWYFGINVSRALMQ
AVSSVKRWKTLSTGRVQGPALAFLVDKELEIRNFVPTPYWVLEALLDNDLIAVHELEKFW
DEEQANKAYEKVKGQKQATVSDVKKSMKTIPPNPPFDLGALQREAHNMFRFSPKKTQEVA
QKLYEKGYCSYPRTSSQKLPDDKAYMDEVLKNLSKNKNYKPYIETILNENRKPISGKNDD
PAHPAVHAVDVPKEKLPDDELKLYELIARRTIALYWDNAKREYSKINLDINGETFKLSGS
RTVEEGWHEIYYYTKFDEVELPELKKNDVINVNNINFEAKETKPPKRYTMASIIKELEKR
KLGTKATRADILEKLTKRGYVIEDGSLTVTDLGIGVTETLRKYCPEIVEETLTRDLEDKL
ELIQDKKIKKEEVIQETKDKLTKILGEFRLKEKEIGTELVDKLDSTNRSLQIIGKCKCGG
DMIIIRTKGKKRFVGCSNYPDCEVTFPLPQKGRIKVLNEVCEKCQNPVIGLDRIKICVNP
DCSTRISEEDKKEIEKAEKEEKICPKCGNKLLIKKGPYGVFRGCENYPKCKYTEKLNGES
NEKEIVGKCPKCGSDLYKRKGRFGEFIGCSNYPKCRHTEKIDKKKEDTPGETKAETKKEA
KPKTTKKAAPKKTATKTTKTTAKKTATKKTTTKKATAKKTAEK