Protein Info for MMP_RS04920 in Methanococcus maripaludis S2

Annotation: CCA tRNA nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 TIGR03671: CCA-adding enzyme" amino acids 13 to 413 (401 residues), 474.7 bits, see alignment E=1.2e-146 PF01909: NTP_transf_2" amino acids 36 to 147 (112 residues), 47.6 bits, see alignment E=2.7e-16 PF09249: tRNA_NucTransf2" amino acids 162 to 282 (121 residues), 139.6 bits, see alignment E=7.8e-45 PF21133: CAA_C" amino acids 307 to 413 (107 residues), 76.1 bits, see alignment E=3.4e-25

Best Hits

Swiss-Prot: 100% identical to CCA_METMP: CCA-adding enzyme (cca) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K07558, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72] (inferred from 100% identity to mmp:MMP0949)

Predicted SEED Role

"tRNA nucleotidyltransferase, archaeal type (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LYP1 at UniProt or InterPro

Protein Sequence (444 amino acids)

>MMP_RS04920 CCA tRNA nucleotidyltransferase (Methanococcus maripaludis S2)
LKKLNADDYIDILNDVLKDICPTDSEKEELKIFSDKIISKIKDISKYPVLDIIQVGSTAR
DANLKNDHDIDIFLRFEKETDRDMLKELGLKLGKDVINYFNGKSWIEYAEHPYVSGEIGK
FNLDIVPCYDIENCEKIISAVDRTPLHNEFLLNAYENNDLSNDIRLLKKFLKGLGIYGSD
LKTAGFSGYLCELLILKYRGFLNLLSAVQTWKPKHSIVLDEIYEMYDLKKDHDFLKFNDS
LVVYDPVDLNRNVAAALNKENLCKFIFYSKMFLKKPSKEFFYGFYKKTTDLLNQRKRGYL
ITLEILRPENIVEDVIYPQMEKIQKSINKLVKEHDFEYLQYQNFADDNTCYLSWEFLIHE
LPDVKLRIGPPVYSEQGVLNFITHNEHYFVKECNVCAYKTRKYKNIQILFEDIVNGKLKN
IITYPKYVCPENAKIRLGTFIERK