Protein Info for MMP_RS04860 in Methanococcus maripaludis S2

Annotation: coenzyme F420-0:L-glutamate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01916: coenzyme F420-0:L-glutamate ligase
  • amino acids 8 to 248 (241 residues), 307.8 bits, E=2.5e-96
• PF01996: F420_ligase
  • amino acids 14 to 225 (212 residues), 243.9 bits, E=6.8e-77

Best Hits

Swiss-Prot: 100% identical to COFE_METMP: Coenzyme F420:L-glutamate ligase (cofE) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K12234, coenzyme F420-0:L-glutamate ligase / coenzyme F420-1:gamma-L-glutamate ligase [EC: 6.3.2.31 6.3.2.34] (inferred from 100% identity to mmp:MMP0937)

MetaCyc: 72% identical to F420-0:gamma-glutamyl ligase subunit (Methanocaldococcus jannaschii)
RXN-8081 [EC: 6.3.2.34]; RXN-8080 [EC: 6.3.2.34, 6.3.2.31]

Predicted SEED Role

"Coenzyme F420-0:L-glutamate ligase @ Coenzyme F420-1:L-glutamate ligase"

MetaCyc Pathways

• factor 420 polyglutamylation (2/2 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.31 or 6.3.2.34

Sequence Analysis Tools

See Q6LYQ1 at UniProt or InterPro

Protein Sequence (248 amino acids)

>MMP_RS04860 coenzyme F420-0:L-glutamate ligase (Methanococcus maripaludis S2)
MIKERVKMEVIGLEIPLISGNEDYTLAELISTYPLEDKDVIVIAETVVSKIEKNVILKNE
ITPSNEAMELSKKLGKEPEVVQVILDESNEIVKLGPNFIITETKHGFVCANSGVDESNTS
KGIKPLPKNPDKSAEEIRMGIEKITGKKVGVIINDSMGRPFRKGSCGIAIGVSGVCGLWD
RKGEKDLFGRELKTTEVGIADELAATASAVMGQSNEGIPLVIIRNAPVPFTNGTGKELIR
KKEEDVFR