Protein Info for MMP_RS04765 in Methanococcus maripaludis S2

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 390 (389 residues), 293.9 bits, E=1.5e-91
• PF13522: GATase_6
  • amino acids 69 to 181 (113 residues), 106.1 bits, E=2.1e-34
• PF13537: GATase_7
  • amino acids 74 to 185 (112 residues), 107.9 bits, E=5e-35
• PF00733: Asn_synthase
  • amino acids 249 to 389 (141 residues), 111.8 bits, E=9e-36
  • amino acids 390 to 482 (93 residues), 78.4 bits, E=1.3e-25

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 100% identity to mmp:MMP0918)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• L-citrulline biosynthesis (4/8 steps found)
• L-glutamate and L-glutamine biosynthesis (3/7 steps found)
• superpathway of L-citrulline metabolism (6/12 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See Q6LYS0 at UniProt or InterPro

Protein Sequence (513 amino acids)

>MMP_RS04765 asparagine synthase (glutamine-hydrolyzing) (Methanococcus maripaludis S2)
MCSISGIIAKDEEGSGSRSLLKNIQKHVINMMKILKHRGPDYSGMMFDDEVLYFENFDDI
TENTETISRMAMGHNRLAIVGTAVQPIPNTDESIWIICNGEIYNHVELRDELSVDHEFKT
DTDSEAIIHTYEDELVDVLDGDYAYAIYDKEKNIIELRRDLMGVKPLYYIDTDEYFAFAS
EKKALYGLLMEINEMDYKSAFNYDISRLNPNSRLIYELDENSWYIEEDLEKVNPDYFEEN
DYELCKNELETTILDSVLKRVKGLERVGIIYSGGVDSTLVSKLASENCDVTLYSVGSANS
EDLIYAERAAKDMGLKFRKKIILEDEFEEYVIKVAKAIDELDVMKLSVGIPIFAASEMAK
EDGIKVVLSGQGADELFAGYNRYQRILNEKGEEGLKKSIISDVFDIHKVNLERDDHCTMA
NGVELRVPFLDKFVIDVGLSIPVEYKIEEPRKKILRDIASKYVPDYIAQRPKKAAQYGSG
SEKMVYAVAKKHGYSKKEINKFFEEYLIEKIKF