Protein Info for MMP_RS03235 in Methanococcus maripaludis S2

Annotation: tRNA pseudouridine(13) synthase TruD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 TIGR00094: tRNA pseudouridine synthase, TruD family" amino acids 34 to 426 (393 residues), 319.8 bits, see alignment E=1.9e-99 PF01142: TruD" amino acids 43 to 337 (295 residues), 306 bits, see alignment E=2.1e-95 amino acids 339 to 425 (87 residues), 74.4 bits, see alignment E=4.2e-25

Best Hits

Swiss-Prot: 100% identical to TRUD2_METMP: Probable tRNA pseudouridine synthase D 2 (truD2) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K06176, tRNA pseudouridine synthase D [EC: 5.4.99.12] (inferred from 100% identity to mmp:MMP0615)

Predicted SEED Role

"tRNA pseudouridine 13 synthase (EC 4.2.1.-)" in subsystem tRNA processing (EC 4.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.-, 5.4.99.12

Use Curated BLAST to search for 4.2.1.- or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6LZL0 at UniProt or InterPro

Protein Sequence (430 amino acids)

>MMP_RS03235 tRNA pseudouridine(13) synthase TruD (Methanococcus maripaludis S2)
MNAPFIQNARKKESEEIINQFRKEKQFKFRNNKIRQKLSEMPININKFILDMERFDGTLK
KYPEDFIVEEITPEGTVLEVGKEIGFEDVEKWHGSFIHFTVEKTNWNTMDALKQIVRATK
TKRKNFGFAGTKDKFAVTTQRFGCFGLKKEQLENINIKDIVIRDVQKTNKKLRMGGLWGN
KFTIKIRDLNLSEKEIKRISDLKLDYVLNYYGIQRFGLIRPITHIVGKFIYERDFESAFY
TYCGTPINETGDSLEARQLVDMGEFKKALKLFNRGHDYEKRLIQQYLKYKDFKMAFTALP
PQLNSMFVNAYQAYLFNEMINKRFDYGFDELEGDILEDNTPTGTLIGYDTKFSGGIQGEI
EKEIVERENLDLKKFKIEDFGNFYGTRRKMVTPIYDFKSRFENEIFELSFKLERGNYATI
VTREFTGNLS