Protein Info for MMP_RS02025 in Methanococcus maripaludis S2

Annotation: DNA-directed DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 784 PF03104: DNA_pol_B_exo1" amino acids 109 to 288 (180 residues), 113.8 bits, see alignment E=1.4e-36 PF13482: RNase_H_2" amino acids 163 to 328 (166 residues), 39.2 bits, see alignment E=1.1e-13 PF00136: DNA_pol_B" amino acids 357 to 762 (406 residues), 258.2 bits, see alignment E=2.1e-80 TIGR00592: DNA polymerase (pol2)" amino acids 389 to 761 (373 residues), 325.7 bits, see alignment E=3.8e-101

Best Hits

KEGG orthology group: K02319, DNA polymerase I [EC: 2.7.7.7] (inferred from 100% identity to mmp:MMP0380)

Predicted SEED Role

"Archaeal DNA polymerase I (EC 2.7.7.7)" in subsystem DNA replication, archaeal (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M091 at UniProt or InterPro

Protein Sequence (784 amino acids)

>MMP_RS02025 DNA-directed DNA polymerase (Methanococcus maripaludis S2)
MESLIDLDYNSDDLCIYLYLINSIIKEKDFKPYFYVNSTDKEQILEFLKDYEKKHKLDSE
ISKMIENIETVKKIVFDENYQEKELSKVTVKYPNNVKTVREILMEFERLYEYDIPFVRRY
LIDNSVIPTSTWDFENNKKIDNKIPDFKTVSFDIEVYCNKEPNPKKDPIIMASFSSKDFN
TVVSTKKFDHEKLEYVKDEKELIKRIIEILKEYDIIYTYNGDNFDFPYLKKRAESFGLEL
KLGKNDEKIKITKGGMNSKSYIPGRVHIDLYPIARRLLNLTKYRLENVTEALFDVKKVDV
GHENIPKMWDNLDETLVEYSHQDAYYTQRIGEQFLPLEIMFSRVVNQSLYDINRMSSSQM
VEYLLLKNSYKMGVIAPNRPSGKEYQKRIRSSYEGGYVKEPLKGIHEDIVSMDFLSLYPS
IIMSHNLSPETIDCTCCSDEENGENEEILGHKFCKKSIGIIPKTLMDLINRRKKVKKVLR
EKAEKGEFDEEYQILDYEQRSIKVLANSHYGYLAFPMARWYSRDCAEITTHLGRQYIQKT
IEEAENFGFKVIYADTDGFYSKWADDKEKLSKYELLEKTREFLKNINNTLPGEMELEFEG
YFKRGIFVTKKKYALIDENEKITVKGLEVVRRDWSNVSKNTQKNVLNALLKEGSVENAKK
VIQDTIKELKDGKVNNEDLLIHTQLTKRIEDYKTTAPHVEVAKKILKSGNRVNTGDVISY
IITSGNKSISERAEILENAKNYDTNYYIENQILPPVIRLMEALGITKDELKDSKKQYTLH
HFLK