Protein Info for MMP_RS01370 in Methanococcus maripaludis S2

Annotation: acetate--CoA ligase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02629: CoA_binding
  • amino acids 9 to 102 (94 residues), 53.5 bits, E=8.5e-18
• PF13380: CoA_binding_2
  • amino acids 11 to 135 (125 residues), 85.7 bits, E=7.9e-28
• PF13607: Succ_CoA_lig
  • amino acids 153 to 288 (136 residues), 176.8 bits, E=4.8e-56
• PF19045: Ligase_CoA_2
  • amino acids 300 to 450 (151 residues), 55.2 bits, E=2.2e-18
• PF13549: ATP-grasp_5
  • amino acids 489 to 697 (209 residues), 184.2 bits, E=6e-58

Best Hits

Swiss-Prot: 53% identical to ACD_METJA: Acetate--CoA ligase [ADP-forming] (MJ0590) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01905, acetyl-CoA synthetase (ADP-forming) [EC: 6.2.1.13] (inferred from 100% identity to mmp:MMP0253)

Predicted SEED Role

"Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

MetaCyc Pathways

• pyruvate fermentation to acetate III (2/2 steps found)
• acetate and ATP formation from acetyl-CoA II (1/1 steps found)
• pyruvate fermentation to acetate and alanine (2/3 steps found)
• methoxylated aromatic compound degradation II (6/9 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (2/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (2/17 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.13

Sequence Analysis Tools

See Q6M0L7 at UniProt or InterPro

Protein Sequence (702 amino acids)

>MMP_RS01370 acetate--CoA ligase family protein (Methanococcus maripaludis S2)
MNTLEGIFNPKSVAVIGASEIEGKVGQSVMKNLLNFKQHGGKVYPINKKYNEVYGIKCYG
SVLDVPETPDLVIISIPAEYAVDAMEECGRKGVKAAIIITAGFAETNNHVLEDRLKAIID
QYGIRTIGPNCLGVINLHNHLNASFSKEFSNMGNIAFISQSGAIMTALLDIANYYNLGFS
KIVSMGNKIDVQEYELLNYLENDPHTKVVALYIEGLKDEKFISAAKKISRKKPVIVLKSG
KSEEGAKAASSHTGSLAGNNAVYDAAFKKSRVFNVESFEDLVNLLKIFSVQPPMKSKKLA
VITNAGGFGVLAADSVEKCGLELADFSATTISELKKYLPDTSGISNPLDLIGDADVNRYK
HAFELVENDPNVDGLLAILTPQGMTDALGVARELVKLKNYMICKKDKIPIVASFVGGTSV
LEARSYLQEKGIPSFICPELAVHALACLYRQSHLMDKYDSPEYLNEIRSEIAEAKANNPE
KIDELLANANESNSKEFLKLNGFAIPEKFVATTKEEAKEYAENLGKVVMKVVSADILHKS
DAGCVIIDPSDASEAFETIMKNGEKYLFDRKIDGIIDGVLIEQFVTGKEIIIGAKRDPVF
GPVVMTGLGGIFVEVLKDVSFGITPITKEYAGEILHSLKSYKILEGVRGENRSDIEFLKE
LIVRVGVLMETYDEISEIDINPAFIKEEGQGGFVGDALIITK