Protein Info for MMP_RS00795 in Methanococcus maripaludis S2

Annotation: formate dehydrogenase H subunit alpha selenocysteine-containing

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04879: Molybdop_Fe4S4
  • amino acids 5 to 58 (54 residues), 77.1 bits, 1.2e-25
• TIGR01591: formate dehydrogenase, alpha subunit
  • amino acids 9 to 673 (665 residues), 882.5 bits, E=8e-270
• PF00384: Molybdopterin
  • amino acids 61 to 478 (418 residues), 266.9 bits, E=4.2e-83
• PF01568: Molydop_binding
  • amino acids 564 to 670 (107 residues), 108.6 bits, E=2.6e-35

Best Hits

Swiss-Prot: 64% identical to FDHA_METJA: Formate dehydrogenase subunit alpha (fdhA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 100% identity to mmp:MMP0138)

MetaCyc: 65% identical to formate dehydrogenase alpha subunit (Methanococcus maripaludis)
RXN-18529 [EC: 1.8.98.6]; 1.2.1.2-RXN [EC: 1.8.98.6, 1.17.1.9]

Predicted SEED Role

"Formate dehydrogenase alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

• coenzyme B/coenzyme M regeneration V (formate-dependent) (1/1 steps found)
• formate oxidation to CO₂ (1/1 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (3/12 steps found)
• purine nucleobases degradation I (anaerobic) (2/15 steps found)
• purine nucleobases degradation II (anaerobic) (5/24 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.1.9 or 1.2.1.2 or 1.8.98.6

Sequence Analysis Tools

See F1SVN3 at UniProt or InterPro

Protein Sequence (676 amino acids)

>MMP_RS00795 formate dehydrogenase H subunit alpha  selenocysteine-containing (Methanococcus maripaludis S2)
MTEFKVVHTICPYCGTGCGIDLVVKDGKVVDSHPFKRHPVNEGKVCIKGNYCYEFVHSED
RLTKPLIKKNGEFIEATWDEALDLIAGKLKQYSPDEVAFFSCARGTNEESYALQKFARTV
LKTNNVDHCARI*HAPTVVGLGECFGSGAMTNSITDLAQADVLLIYGSNTFEAHPLIARS
IVKAKENGTKIIAIDPRTTHTAKMADLHLKLIPGSNIDLINTITNIIIQEGMADEEFIKN
RTEGYDELKDVVSKYTLEKTAELSGIPAETILEAARMYGSAENASIMYCLGVTEYTFGVD
NVKSCCNLAMVTGNLGRPGTGVNPLRGQNNVQGACDMGALPNVFPGYQKVGEAYERLENL
WETADLNREIGLTSPEVLHKAGEQVKFLHIVGEDPMVADADINHVEKALKSLDFFVVQDI
FLTETAKLADVVLPAACWAEKDGTFTNSERRVQRIRKAVDAPGDALPDWLIVRKLAEKMG
AGEKLNFESASEIFDEMAKVIPQYAGMSFERLGIDGLQWPCKTPEDPGTPILHKEKFLRP
NGLGKFTPVEHKDADELIDEEYPLILTTGRIIFHYNSGTMTRRCDSITNEIDENFIEINT
EDAKELGIKPGEKVRVSSRRGTVNADARVTENVIKGVVYMSFHFLEEATNKLTNSAYDPV
SKTAELKICAVKVEKI