Protein Info for MMP_RS00505 in Methanococcus maripaludis S2

Annotation: bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01470: siroheme synthase, N-terminal domain
  • amino acids 2 to 191 (190 residues), 75.4 bits, E=2.5e-25
• PF13241: NAD_binding_7
  • amino acids 4 to 117 (114 residues), 69.3 bits, E=2e-23

Best Hits

KEGG orthology group: K02304, precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 4.99.1.4] (inferred from 100% identity to mmp:MMP0089)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.3.1.76, EC 4.99.1.4)

MetaCyc Pathways

• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (14/15 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• siroheme biosynthesis (4/4 steps found)
• factor 430 biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.76 or 4.99.1.4

Sequence Analysis Tools

See Q6M129 at UniProt or InterPro

Protein Sequence (201 amino acids)

>MMP_RS00505 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase (Methanococcus maripaludis S2)
MIPVFLDLGGFNVLIFGFGSVGKRRFEKFLKSRAKITVYSKCIDKNDISKYKNAIFFESD
VTNLSDEELYSIIKNHDIIITAIDEENNKRIVSVAKSLKKFINSSTFEKESNLIVPACCE
VDGVSFAVYTGGKSPIIAREIRKNVEEYLKNSEFELDFQNKLRDFLKSEIEDQNERKKVL
ETVFSDKKFKKELLKLIEKHR