Protein Info for MMP_RS00420 in Methanococcus maripaludis S2

Annotation: (4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 326 TIGR03123: probable H4MPT-linked C1 transfer pathway protein" amino acids 3 to 322 (320 residues), 423.7 bits, see alignment E=2.6e-131 PF01968: Hydantoinase_A" amino acids 53 to 323 (271 residues), 153.9 bits, see alignment E=2.8e-49

Best Hits

Swiss-Prot: 60% identical to MFNF_METJA: (4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase (mfnF) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K07072, (no description) (inferred from 100% identity to mmp:MMP0072)

MetaCyc: 60% identical to (4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase (Methanocaldococcus jannaschii)
RXN-15945 [EC: 2.5.1.131]

Predicted SEED Role

"Uncharacterized protein MJ0840"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.131

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q6M145 at UniProt or InterPro

Protein Sequence (326 amino acids)

>MMP_RS00420 (4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase (Methanococcus maripaludis S2)
MILGIDIGGANTKITELHENGEFKVHHLYFPMWKNNDKLAEVLKTYSKDISHVALVTTAE
LADSYETKKEGVDNILNAAESAFGSNISVFDSDGNFISLEGAKTNYMKVSASNWCGTAKW
VSKNIEENCILVDMGSTTTDIIPIVDGKVVAEKTDLERLMNHELLYVGTLRTPISHLGNT
ILFKGVNTNVSSEYFAITADISVVLEKVTTEEYTCDTPDGKGTDKRSSLVRISKVLCSDL
DQISEIDAENIAKTYYELWKELILENVKKVAEKYGSKKVVITGIGENILKDALGDFEVIS
VAERYGKDVSLATPSFAVAELLKNEL