Protein Info for MMP_RS00045 in Methanococcus maripaludis S2

Annotation: 2-oxoacid:acceptor oxidoreductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01855: POR_N
  • amino acids 15 to 246 (232 residues), 249.1 bits, E=4.9e-78
• PF17147: PFOR_II
  • amino acids 270 to 359 (90 residues), 63.9 bits, E=1.6e-21

Best Hits

Swiss-Prot: 75% identical to KORA_METJA: 2-oxoglutarate synthase subunit KorA (korA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K00174, 2-oxoglutarate ferredoxin oxidoreductase subunit alpha [EC: 1.2.7.3] (inferred from 100% identity to mmp:MMP0003)

MetaCyc: 100% identical to 2-oxoglutarate ferredoxin oxidoreductase alpha subunit (Methanococcus maripaludis)
2-oxoglutarate synthase. [EC: 1.2.7.3]

Predicted SEED Role

"2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3)" (EC 1.2.7.3)

MetaCyc Pathways

• incomplete reductive TCA cycle (6/7 steps found)
• reductive TCA cycle I (6/11 steps found)
• TCA cycle V (2-oxoglutarate synthase) (4/9 steps found)
• reductive TCA cycle II (5/12 steps found)
• TCA cycle VI (Helicobacter) (2/9 steps found)
• TCA cycle VII (acetate-producers) (2/9 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (35/56 steps found)

KEGG Metabolic Maps

• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 1.2.7.3

Use Curated BLAST to search for 1.2.7.3

Sequence Analysis Tools

See Q6M1B3 at UniProt or InterPro

Protein Sequence (363 amino acids)

>MMP_RS00045 2-oxoacid:acceptor oxidoreductase subunit alpha (Methanococcus maripaludis S2)
MTKVNFMQGNMACVEGAIKAGCKFFGGYPITPSTEIAEGMAKRLPKIGGFYSQMEDEIAS
IASIIGASWAGTKSMTATSGPGMSLMLENIGYAYMTETPCVLVNVQRGGPSTGQPTAAAQ
GDMMQVRWGSHGDYEPIALCPSSVQEMYDFTILAFNYAEKYRIPVFVMADEILGHMREKV
VLHDDIEIFNREKPEEKPCTKPYPFDKEVPPMPTFGEGYNVHVTGLTHGENGYPDVSAET
HDKLVRRICNKILENKDDIVLYEGKYLESETMFICYGTPSRTVKHTVESLREQGQDVGYI
RLKTVFPFPDKLIAGLKASKIIVPEMNLGQISGEVMKYAKCEVVGCGKIGGELHRPEDLK
ELI