Protein Info for MMJJ_RS09195 in Methanococcus maripaludis JJ

Annotation: tryptophan synthase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00290: Trp_syntA
  • amino acids 2 to 248 (247 residues), 256.5 bits, E=2.1e-80
• TIGR00262: tryptophan synthase, alpha subunit
  • amino acids 3 to 246 (244 residues), 286 bits, E=9.1e-90
• PF00977: His_biosynth
  • amino acids 166 to 224 (59 residues), 27.8 bits, E=1.7e-10

Best Hits

Swiss-Prot: 96% identical to TRPA_METMP: Tryptophan synthase alpha chain (trpA) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K01695, tryptophan synthase alpha chain [EC: 4.2.1.20] (inferred from 96% identity to mmp:MMP1002)

MetaCyc: 51% identical to tryptophan synthase subunit alpha (Thermococcus kodakarensis)
Tryptophan synthase. [EC: 4.2.1.20]; Indole-3-glycerol-phosphate lyase. [EC: 4.2.1.20, 4.1.2.8]

Predicted SEED Role

"Tryptophan synthase alpha chain (EC 4.2.1.20)" in subsystem Auxin biosynthesis or Tryptophan synthesis (EC 4.2.1.20)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (17/18 steps found)
• superpathway of L-tryptophan biosynthesis (12/13 steps found)
• L-tryptophan biosynthesis (6/6 steps found)
• DIBOA-glucoside biosynthesis (1/6 steps found)
• superpathway of benzoxazinoid glucosides biosynthesis (1/13 steps found)
• superpathway of chorismate metabolism (25/59 steps found)

KEGG Metabolic Maps

• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.20

Use Curated BLAST to search for 4.1.2.8 or 4.2.1.20

Protein Sequence (258 amino acids)

>MMJJ_RS09195 tryptophan synthase subunit alpha (Methanococcus maripaludis JJ)
MNKPVLVSFLVSGDPNPDATLKFMKTLDKYSGVIELGIPFSDPVADGPTIQAADVRSLSN
KFKISKSFDILKEFRKESSTPVILMTYYNPVFKRGIENFVIQAKEAGANGLIIVDLPLQE
ATEYREICKKHEMGTVFLAAPNTPEERLKISDEASTEFLYLISTFGITGARDTFEQMTFD
FIKRARTTCKGKICVGFGISKGSHAESLIEQGADGVIVGSAFVDIIKNYGDSEEALVKLE
ELAKELSEGIDKGYGKRK