Protein Info for MMJJ_RS08715 in Methanococcus maripaludis JJ

Annotation: NAD-dependent epimerase/dehydratase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 1 to 189 (189 residues), 54.6 bits, E=3.6e-18
• PF05368: NmrA
  • amino acids 2 to 115 (114 residues), 31.1 bits, E=7.7e-11
• PF02719: Polysacc_synt_2
  • amino acids 3 to 244 (242 residues), 53.9 bits, E=6.5e-18
• PF01370: Epimerase
  • amino acids 3 to 229 (227 residues), 211.6 bits, E=4.7e-66
• PF16363: GDP_Man_Dehyd
  • amino acids 4 to 290 (287 residues), 178.5 bits, E=1.1e-55
• PF01073: 3Beta_HSD
  • amino acids 4 to 220 (217 residues), 100.1 bits, E=4.7e-32
• PF07993: NAD_binding_4
  • amino acids 5 to 214 (210 residues), 39.3 bits, E=1.8e-13
• PF13460: NAD_binding_10
  • amino acids 7 to 166 (160 residues), 39.3 bits, E=2.7e-13

Best Hits

Swiss-Prot: 65% identical to GALE_METJA: Putative UDP-glucose 4-epimerase (MJ0211) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 85% identity to mmx:MmarC6_1577)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose detoxification (1/3 steps found)
• D-galactose degradation I (Leloir pathway) (2/5 steps found)
• colanic acid building blocks biosynthesis (6/11 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (3/7 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (2/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (10/33 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.2

Protein Sequence (291 amino acids)

>MMJJ_RS08715 NAD-dependent epimerase/dehydratase family protein (Methanococcus maripaludis JJ)
MKILVTGGAGFIGSHIVDILIENGHDVSILDNLSTGNEKNLNSKAKFIKGDILDKNLDLT
GFDCVIHEAAQINVRTSIEDPVFDANINILGTVNILEKMKENGVKKIIFSSSVATVGEPE
YLPVDENHTLKPLSPYGLSKVCAEEYIKLYNKFYGTEYCILRYANVYGERQDPLGEAGVI
SIFIDKMKKGEQPVIYGDGNQTRDFVNVKDVARANLMALNWKNETVNICSGKEISINELF
KIISSEHGFDLNPIYEEGMDGEIYQIYMENDNAKSLGWVPEVELENGIKEI