Protein Info for MMJJ_RS08035 in Methanococcus maripaludis JJ

Annotation: cobalt-precorrin-7 (C(5))-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 PF00590: TP_methylase" amino acids 13 to 205 (193 residues), 115.7 bits, see alignment E=1.4e-37 TIGR02467: precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit" amino acids 15 to 216 (202 residues), 165.5 bits, see alignment E=5.4e-53

Best Hits

Swiss-Prot: 60% identical to CBIE_METJA: Probable cobalt-precorrin-7 C(5)-methyltransferase (cbiE) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K03399, precorrin-6Y C5,15-methyltransferase [EC: 2.1.1.132] (inferred from 92% identity to mmp:MMP1227)

Predicted SEED Role

"Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-)" in subsystem Coenzyme B12 biosynthesis (EC 2.1.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-, 2.1.1.132

Use Curated BLAST to search for 2.1.1.- or 2.1.1.132

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (219 amino acids)

>MMJJ_RS08035 cobalt-precorrin-7 (C(5))-methyltransferase (Methanococcus maripaludis JJ)
MFFKKNYKWDTVIYIVGIGPGSRDYVTEKAVDTVKTSDFVLGSKRSLEIFETNGKKIELT
VNLKNELYEFLKNYKNSNPKEKVSILSTGDPCFSGLLKTISSFDFIKKEDFEVIPGISSI
QMAAAKAKISWEDYNILTIHGKEENLKKLLDYVKNDEKVIFLPNNLKNDLKFLIENGISD
EKEITVLENLSYSNERIITDKISNLMENDYSYLLVCIIN