Protein Info for MMJJ_RS07380 in Methanococcus maripaludis JJ

Annotation: sulfide-dependent adenosine diphosphate thiazole synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 TIGR00292: thiazole biosynthesis enzyme" amino acids 9 to 259 (251 residues), 340 bits, see alignment E=3.6e-106 PF01946: Thi4" amino acids 13 to 241 (229 residues), 275.2 bits, see alignment E=1.7e-85 PF01494: FAD_binding_3" amino acids 29 to 62 (34 residues), 36.1 bits, see alignment 2e-12 PF01266: DAO" amino acids 31 to 74 (44 residues), 32.3 bits, see alignment 3.6e-11 PF00890: FAD_binding_2" amino acids 31 to 68 (38 residues), 26.8 bits, see alignment 1.4e-09 PF07992: Pyr_redox_2" amino acids 31 to 129 (99 residues), 30.4 bits, see alignment E=1.2e-10 PF12831: FAD_oxidored" amino acids 31 to 175 (145 residues), 39.3 bits, see alignment E=2.4e-13 PF03486: HI0933_like" amino acids 31 to 90 (60 residues), 27.4 bits, see alignment E=6.7e-10 PF13450: NAD_binding_8" amino acids 34 to 66 (33 residues), 33 bits, see alignment 2.7e-11

Best Hits

Swiss-Prot: 99% identical to THI4_METMP: Thiamine thiazole synthase (thi4) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K03146, thiamine biosynthetic enzyme (inferred from 99% identity to mmp:MMP1352)

MetaCyc: 72% identical to sulfide-dependent adenosine diphosphate thiazole synthase (Methanocaldococcus jannaschii)
RXN-19351 [EC: 2.4.2.59]

Predicted SEED Role

"Thiazole biosynthetic enzyme Thi4 / Ribose 1,5-bisphosphate or 5-ribose-1,2-cyclic phosphate dehydrogenase" in subsystem Calvin-Benson cycle or Thiamin biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (262 amino acids)

>MMJJ_RS07380 sulfide-dependent adenosine diphosphate thiazole synthase (Methanococcus maripaludis JJ)
MDGKLRADEVAVTKSILKSTFDMWMDLIDVDVVIVGAGPSGLTAAKYLAQNGVKTVVLER
HLSFGGGTWGGGMGFPNIVVEKPADEILREAGIKLDEVIGEPELFTADSVEVPAKLGVAA
IDAGAKILTGIVVEDLILKEDKVSGVVIQSYSIEKAGLHVDPITISAKYVIDSTGHDSSV
IHTLARKNKDLGIEVPGEKSMWADKGENSLTRNTREVFPGLYVCGMAANAYHAGYRMGAI
FGGMYLSGKKCAELILEKLENK