Protein Info for MMJJ_RS06670 in Methanococcus maripaludis JJ

Annotation: phenylalanine--tRNA ligase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 TIGR00468: phenylalanine--tRNA ligase, alpha subunit" amino acids 180 to 496 (317 residues), 392.9 bits, see alignment E=6e-122 PF01409: tRNA-synt_2d" amino acids 219 to 495 (277 residues), 306 bits, see alignment E=3e-95 PF17759: tRNA_synthFbeta" amino acids 332 to 464 (133 residues), 28.9 bits, see alignment E=1.2e-10

Best Hits

Swiss-Prot: 97% identical to SYFA_METMP: Phenylalanine--tRNA ligase alpha subunit (pheS) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K01889, phenylalanyl-tRNA synthetase alpha chain [EC: 6.1.1.20] (inferred from 97% identity to mmp:MMP1496)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (501 amino acids)

>MMJJ_RS06670 phenylalanine--tRNA ligase subunit alpha (Methanococcus maripaludis JJ)
MELHNDEKRLLKAFQESNKKTMNLEELSKYIEKEKVMRAAFWLSGRDFLEIIENKTKLCE
LTKLGKNSIDFGIPERKVANYIKENNLESIPIKELSKILEKDETGAALGNLKKKGLVEID
KGNIVFKNLDYEDIEEEILKKVSINPDLSIYSKDEVKIVENLKKRGFLNLKEVVDRSFEI
KDAGTEFIKNPIEIKEEITQLTREMIVSGKWKDYFIRPYDAKIPTEDVYPAKAHPMSKMI
QEVNEVLISMGFKEVKSQIVQTEFWNFDTLFEPQDHPARDMQDTFFVKYPNTGKVPENLL
EKVKGIHECGTIDGEKISKGWCYKFDENVSKRTVLRTHTTVSSIKYLASLSESEREKPHK
VFCIDRVFRNETIDYKHLPEFYQCEGIVMAEDVNFDNLVGILKEFLRKLGFEKVRIRPAY
FPFTEPSLEAEVYMEGKGWLELLGAGVFRPEVLEPFGIKKPVLAWGIGLSRLAMLRLGLT
DIRELHKNDMEWLKKTAVNEQ