Protein Info for MMJJ_RS06265 in Methanococcus maripaludis JJ

Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00155: Aminotran_1_2
  • amino acids 30 to 364 (335 residues), 187.3 bits, E=4.9e-59
• PF01053: Cys_Met_Meta_PP
  • amino acids 67 to 200 (134 residues), 27.2 bits, E=1.5e-10

Best Hits

Swiss-Prot: 71% identical to BIOF_METVS: Putative 8-amino-7-oxononanoate synthase (bioF) from Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 95% identity to mmp:MMP1574)

MetaCyc: 45% identical to BioF (Lysinibacillus sphaericus)
8-amino-7-oxononanoate synthase. [EC: 2.3.1.47]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

• biotin biosynthesis II (5/6 steps found)
• 8-amino-7-oxononanoate biosynthesis III (2/2 steps found)
• 8-amino-7-oxononanoate biosynthesis II (1/2 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (1/5 steps found)
• biotin biosynthesis I (5/15 steps found)
• 8-amino-7-oxononanoate biosynthesis I (1/11 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.47

Protein Sequence (370 amino acids)

>MMJJ_RS06265 8-amino-7-oxononanoate synthase (Methanococcus maripaludis JJ)
MFRENLKKELDLIKNQNLYRKLKLEDNIRLNFSKNDYLGLSKNNEVINAYNDGLNYGAGA
TGSRLTSGNINHEELEETISEFKKTEKSLVYSSGYGTNLGVISSLCKKGDLVLSDELNHA
SIVDGIRLSRSDKKIYSHNNMQELIEILENSRNYENKFIVSDAVFSMDGDIAPVDELKKI
ADEYNAVLILDDAHGTGVLGNGKGTLHHFKIKPTDNIIQIGTLSKAVGTVGGFVCGIEEL
IDYLINTSRSFIYSTALPPAVISASIKSFELIKSGELTAKLSKNINTANKIFKSSGFIEE
EKITPIYPFIFGKDSIKISEELLKYKIFCVGIRYPTVKRGFERIRLSITAENKKEDFEYL
CECISKIKNG