Protein Info for MMJJ_RS05650 in Methanococcus maripaludis JJ

Annotation: formylmethanofuran dehydrogenase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to FWDB_METMP: Tungsten-containing formylmethanofuran dehydrogenase 2 subunit B (fwdB) from Methanococcus maripaludis (strain S2 / LL)

KEGG orthology group: K00201, formylmethanofuran dehydrogenase subunit B [EC: 1.2.99.5] (inferred from 100% identity to mmp:MMP1691)

MetaCyc: 48% identical to formylmethanofuran dehydrogenase subunit B (Archaeoglobus fulgidus DSM 4304)
FORMYLMETHANOFURAN-DEHYDROGENASE-RXN [EC: 1.2.7.12]

Predicted SEED Role

"Formylmethanofuran dehydrogenase (tungsten) subunit B (EC 1.2.99.5) @ selenocysteine-containing" (EC 1.2.99.5)

MetaCyc Pathways

gluconeogenesis II (Methanobacterium thermoautotrophicum) (16/18 steps found)
methanogenesis from H₂ and CO₂ (6/6 steps found)
methyl-coenzyme M oxidation to CO₂ II (6/6 steps found)
methyl-coenzyme M oxidation to CO₂ I (5/6 steps found)
reductive acetyl coenzyme A pathway II (autotrophic methanogens) (5/6 steps found)
methoxylated aromatic compound degradation II (6/9 steps found)
superpathway of methanogenesis (11/21 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (34/56 steps found)

KEGG Metabolic Maps

Folate biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.5

Use Curated BLAST to search for 1.2.7.12 or 1.2.99.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>MMJJ_RS05650 formylmethanofuran dehydrogenase subunit B (Methanococcus maripaludis JJ)
MEVFKNVVCPFCGTLCDDIEVLVENNHVVGTRNACRIGNAKFMHFEGAVRHESPLMRENK
KDDFKKVDYETATEETARLLVEAKLPLIYGWSSAECHAQQLGVLLAEKTKAIVDNTASVU
HGPSLLAVQDVGYPVSTLGETKNRADVVLFWGSNPMHAHPRHMSRYSVFPRGFFRQRGKQ
DRQMIVVDPRKTDTAKLADIHLQVEPHKDYELVSALRAAAKGFNIEAEQVAGVPTETIYE
AVDICKNAQFGSLFFAMGVTMSRGKHRIIDNAIQFVIDMNAYTKFVLTPMRGHYNVNGFN
QVSTWVTGYPYGVDFSRGYPRYNPGETASNDVLQRGDTDMMINVASDAGAHFPQKAVQHM
AKIPLVCIDPHETPSSVISNIVLPPAITGLEVSGTAYRMDGVPIELRKVIKAPEGMLSDA
EIMKMLIKKVDEMK