Protein Info for MMJJ_RS04825 in Methanococcus maripaludis JJ

Annotation: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01676: Metalloenzyme
  • amino acids 1 to 396 (396 residues), 151.4 bits, E=5.1e-48
• TIGR00306: phosphoglycerate mutase (2,3-diphosphoglycerate-independent), archaeal form
  • amino acids 4 to 396 (393 residues), 385.2 bits, E=2.1e-119
• PF10143: PhosphMutase
  • amino acids 37 to 216 (180 residues), 143.8 bits, E=7.7e-46

Best Hits

Swiss-Prot: 56% identical to APGM2_METJA: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (apgM2) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01834, phosphoglycerate mutase [EC: 5.4.2.1] (inferred from 96% identity to mmp:MMP0112)

Predicted SEED Role

"2,3-bisphosphoglycerate-independent phosphoglycerate mutase, archaeal type (EC 5.4.2.1)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 5.4.2.1)

MetaCyc Pathways

• gluconeogenesis II (Methanobacterium thermoautotrophicum) (16/18 steps found)
• glycolysis V (Pyrococcus) (10/10 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• Rubisco shunt (8/10 steps found)
• glycolysis IV (8/10 steps found)
• gluconeogenesis I (10/13 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• glycolysis III (from glucose) (8/11 steps found)
• homolactic fermentation (8/12 steps found)
• Bifidobacterium shunt (10/15 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• superpathway of anaerobic sucrose degradation (12/19 steps found)
• superpathway of glucose and xylose degradation (10/17 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (10/17 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (4/9 steps found)
• superpathway of hexitol degradation (bacteria) (10/18 steps found)
• glycerol degradation to butanol (8/16 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (12/22 steps found)
• photorespiration II (3/10 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (9/19 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (14/26 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (14/27 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (13/26 steps found)
• ethene biosynthesis V (engineered) (12/25 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (34/56 steps found)
• superpathway of N-acetylneuraminate degradation (9/22 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

Compare fitness of predicted isozymes for: 5.4.2.1

Use Curated BLAST to search for 5.4.2.1

Protein Sequence (426 amino acids)

>MMJJ_RS04825 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (Methanococcus maripaludis JJ)
MRTLLLLLDGLGDRPSEVLKDKTPLEYAKTPNLDSFAKKSVTGMMTPYKKGVPLGTEVAH
FLLWGYDLSEFPGRGIIEALGEDIKLKDNSIYLRATFGYVKRKNGFFVKDRRTKNISIEE
IKQLINSLPKKIGDYEFNLHYSFDTHCILEISSSNYEISDKISDSDPFYRERHVLKVKPL
KNISERIEIENAVKTAEVLNKYLLKCSEILENHPVNITRKENNSQLANFLLTKWAGRYRE
IDSFHDKWGMNAAIIAKSSVFKGLSKLLKMDYYPEGDFQKAFLKGIELKNYDFVHIHTKE
PDEAGHTKNPINKVEVIEKIDKCLQKINELNDELIIITADHSTPSVGTLIHSGEDVPLAI
FKNGIMRDRVEYFNEKECSKGQLNIKSKDLMNLILNDTNKTRPYTTRIGNKLLNYIPENN
SIEHLK