Protein Info for MMJJ_RS04690 in Methanococcus maripaludis JJ

Annotation: Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04422: FrhB_FdhB_N
  • amino acids 4 to 77 (74 residues), 75.4 bits, E=6.5e-25
• PF04432: FrhB_FdhB_C
  • amino acids 87 to 238 (152 residues), 147.9 bits, E=4.2e-47
• PF00037: Fer4
  • amino acids 329 to 342 (14 residues), 22.3 bits, see alignment (E = 1.8e-08)

Best Hits

Swiss-Prot: 68% identical to FDHB_METJA: Formate dehydrogenase subunit beta (fdhB) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K00125, formate dehydrogenase, beta subunit [EC: 1.2.1.2] (inferred from 100% identity to mmp:MMP0139)

MetaCyc: 63% identical to formate dehydrogenase beta subunit (Methanococcus maripaludis)
RXN-18529 [EC: 1.8.98.6]; 1.2.1.2-RXN [EC: 1.8.98.6, 1.17.1.9]

Predicted SEED Role

"Formate dehydrogenase beta subunit (EC 1.2.1.2)" in subsystem Formate dehydrogenase (EC 1.2.1.2)

MetaCyc Pathways

• coenzyme B/coenzyme M regeneration V (formate-dependent) (1/1 steps found)
• formate oxidation to CO₂ (1/1 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (3/12 steps found)
• purine nucleobases degradation I (anaerobic) (2/15 steps found)
• purine nucleobases degradation II (anaerobic) (5/24 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.1.9 or 1.2.1.2 or 1.8.98.6

Protein Sequence (375 amino acids)

>MMJJ_RS04690 Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain (Methanococcus maripaludis JJ)
MSYYLVQSTDEDILKHAECGGAVTAFFKYLLDKKLVDGVLALKKGEDVYDGLPYLVNDSK
ELVETCGSLHCAPTMFGNMISKHLKDMNLAVSVKPCDAMAIVELEKRHQIDKDKLYTIGL
NCGGTVPPQTAKKMIELFYDVDPEDVIKEEIDKGKFIIELKDGSEKSVKIDELEEEGYGR
RTNCQRCELKVPRNSDLACGNWGTEKGWTFVEVGSEKGEELLKNAQKEGYINVKAPSEKA
LEIRGKIEKSMINLGKKFQKEQLDEKYPSPEKWDEYWSRCIKCYGCRDVCPICFCKECAL
GEDYLDKGTIPPDPIMFQGIRLSHMSFSCINCGQCEDVCPVEIPLAKIYHRAQLKIRETT
GFVPGIDDSMPFLYK