Protein Info for MMJJ_RS04450 in Methanococcus maripaludis JJ

Annotation: diphthamide biosynthesis enzyme Dph2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 TIGR00322: diphthamide biosynthesis enzyme Dph1/Dph2 domain" amino acids 3 to 292 (290 residues), 230 bits, see alignment E=4.7e-72 TIGR03682: diphthamide biosynthesis enzyme Dph2" amino acids 17 to 303 (287 residues), 300.9 bits, see alignment E=1e-93 PF01866: Diphthamide_syn" amino acids 25 to 295 (271 residues), 186.5 bits, see alignment E=3.9e-59

Best Hits

Swiss-Prot: 54% identical to DPH2_METJA: 2-(3-amino-3-carboxypropyl)histidine synthase (dph2) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K07561, diphthamide synthase subunit DPH2 (inferred from 97% identity to mmp:MMP0188)

Predicted SEED Role

"diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (309 amino acids)

>MMJJ_RS04450 diphthamide biosynthesis enzyme Dph2 (Methanococcus maripaludis JJ)
MWDLETDKVLNEIKTKNARKVLFQAPEGLKRAVEKEIEFLKTKIDSELMIWGETCFGACD
LCDEEVKILGIDLIIHYGHEELSYVHSEIPVVFVHAYFKENEYFLKDVENILEKMENSTV
TTTIQFKKALSKFNPVVILGCRAPVENAENVLFVGTGRFHPLMMAYKLKKTVRIYNPITR
EISEISDQEIKKLIKLRIGRVSKLLLNPPKKIGVVLSTKKGQCRLKSFENVIELLKKNNI
EYIPIILNNVSQSYLFYKVDAYVICACPRIVMDDYQNYESTLITPEELRMYLSNDFEYKF
DEILENNFY