Protein Info for MMJJ_RS01600 in Methanococcus maripaludis JJ

Annotation: radical SAM protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 PF04055: Radical_SAM" amino acids 33 to 215 (183 residues), 69 bits, see alignment E=2.9e-23

Best Hits

Swiss-Prot: 64% identical to NIFB_METJA: FeMo cofactor biosynthesis protein NifB (nifB) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K02585, nitrogen fixation protein NifB (inferred from 98% identity to mmp:MMP0658)

Predicted SEED Role

"Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (archaea)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (297 amino acids)

>MMJJ_RS01600 radical SAM protein (Methanococcus maripaludis JJ)
MKEDKMSKFAHITSVHPCYNEKLHHKIGRVHLPVAPKCNIACRFCKRSIGDESVCEDRPG
VSHHIMKPAEVENYLNDLLERSPNIKVAGIAGPGDSLFNEETFETLEVLKEKFPDLVRCL
STNGLLLPKYAERLAELGVKTVTVTVNAVDPKIQAQIVDWIYYEGKVYRGEEGAEILIKN
QIEGVKKLAEFDVAVKINTVLIPEINMDHIVEIAKTFEKDAFVHNVIPLIPMYKMENLRK
PTCDEISNIRDTAEEYLHQFRACQQCRADAAGLITEHKHLGEKGKKLDIYDLKHFSH