Protein Info for LRK55_RS18725 in Rhodanobacter denitrificans MT42

Annotation: cation diffusion facilitator family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 320 transmembrane" amino acids 29 to 51 (23 residues), see Phobius details amino acids 60 to 79 (20 residues), see Phobius details amino acids 98 to 118 (21 residues), see Phobius details amino acids 131 to 151 (21 residues), see Phobius details amino acids 172 to 189 (18 residues), see Phobius details amino acids 195 to 213 (19 residues), see Phobius details TIGR01297: cation diffusion facilitator family transporter" amino acids 26 to 299 (274 residues), 239.4 bits, see alignment E=2.4e-75 PF01545: Cation_efflux" amino acids 30 to 220 (191 residues), 145.3 bits, see alignment E=1.1e-46

Best Hits

Swiss-Prot: 38% identical to CZCD_BACVZ: Cadmium, cobalt and zinc/H(+)-K(+) antiporter (czcD) from Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42)

KEGG orthology group: K03295, cation efflux system protein, CDF family (inferred from 66% identity to mab:MAB_0183c)

MetaCyc: 35% identical to Zn2+/Cd2+/Ni2+/Cu2+ exporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-200

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein CzcD" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (320 amino acids)

>LRK55_RS18725 cation diffusion facilitator family transporter (Rhodanobacter denitrificans MT42)
MSTSEPHKHDHDHHHDHSHGVAPDANKKYLTIALLLLLGFMAVEVVVGMLAKSLALISDA
GHMLTDVGSIGLALVAMRLAKRPASGSYTFGLKRVEILSAQANGLTLLLLSVWFIVEAIR
RLIDPPVVEGLLVTVIAVIGIGVNLLAVWAMSKANRQSLNVEGSFQHILTDLYAFIATAI
AGGIIWWTGWNRVDSIAALVVAGLMLKAGIGLVRESGRIFLEAAPRGLDPVTIAEAIRAL
PAVTRLDDLHVWEVTSGMPALSGHIYVNHDIDCHDVRRAVEAMLHDRFKITHTTLQTDHG
ATDESASAAGCSFASTGGAH