Protein Info for LRK55_RS16670 in Rhodanobacter denitrificans MT42

Annotation: translation elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01393: elongation factor 4
  • amino acids 3 to 596 (594 residues), 964.5 bits, E=1.9e-294
• PF00009: GTP_EFTU
  • amino acids 4 to 181 (178 residues), 183.6 bits, E=7.3e-58
• TIGR00231: small GTP-binding protein domain
  • amino acids 7 to 174 (168 residues), 58.9 bits, E=5.5e-20
• PF03144: GTP_EFTU_D2
  • amino acids 205 to 275 (71 residues), 45.7 bits, E=1.8e-15
• PF00679: EFG_C
  • amino acids 400 to 478 (79 residues), 63.5 bits, E=3.8e-21
• PF06421: LepA_C
  • amino acids 488 to 594 (107 residues), 171.6 bits, E=1.1e-54

Best Hits

Swiss-Prot: 86% identical to LEPA_XANAC: Elongation factor 4 (lepA) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 87% identity to psu:Psesu_1990)

MetaCyc: 72% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (597 amino acids)

>LRK55_RS16670 translation elongation factor 4 (Rhodanobacter denitrificans MT42)
MELIRNFSIIAHIDHGKSTLADRIIHLCGGLADREMEAQVLDNNPIERERGITIKAQSVS
LPYTARDGKTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAV
EMGLEVVPVLNKIDLPTADIEKVKGEIEAVIGIDATDAVAISAKTGLNVVEVLEAIVARI
PPPKPRDTDRLQALIIDSWFDNYLGVVSLVRVMQGEIKPGDKLLVMSTGRTHEVDDVGVF
TPKRKKLDKLGAGEVGWINASIKDVHGAPVGDTLTLAGKPADKALPGFQTMQPRVFAGLF
PVSSDDFPAMREALDKLRLNDAALFFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYD
LDLITTAPTVVYEVLKTDGSTMMLDNPAKLPTNSSLVQEIREPIIVANILTPPDYIGNVI
TLCEEKRGVQRSIQYLATQVQISYEMPLAEVVMDFFDRLKSVSRGYASMDYHFERFDAGP
FVRVDVLINGDRVDALSLIVHRSHADRRGRDLVERMKDLIPRQQFDVAIQAAVGAQIIAR
STVKALRKNVLAKCYGGDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLKVDK