Protein Info for LRK55_RS16070 in Rhodanobacter denitrificans MT42

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 556 TIGR01228: urocanate hydratase" amino acids 11 to 555 (545 residues), 989.5 bits, see alignment E=2.2e-302 PF17391: Urocanase_N" amino acids 13 to 139 (127 residues), 209.1 bits, see alignment E=2.3e-66 PF01175: Urocanase" amino acids 142 to 350 (209 residues), 291.1 bits, see alignment E=7.4e-91 PF17392: Urocanase_C" amino acids 353 to 547 (195 residues), 312.3 bits, see alignment E=1.7e-97

Best Hits

Swiss-Prot: 83% identical to HUTU_PSEA7: Urocanate hydratase (hutU) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 83% identity to psu:Psesu_1929)

MetaCyc: 82% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (556 amino acids)

>LRK55_RS16070 urocanate hydratase (Rhodanobacter denitrificans MT42)
MNAPTRIDTTRTIRAPRGSELSCKSWLSEAPFRMLQNNLDPEVAENPAELVVYGGIGRAA
RNWECFDAILKALRELNDDETLLVQSGKPVGVFRTHADAPRVLIANSNLVPHWATWEHFN
ELDKKGLMMYGQMTAGSWIYIGSQGIIQGTYETFVEMGRQSYGGKLKGKWLLTAGLGGMG
GAQPLAASLAGMCSLNIECQQSRIDFRLKTRYVDEQASDLDDALARIAKYTAAGEAKSIA
LLGNAADVLPELVRRGVRPDAVTDQTSAHDPVNGYLPQGWTVEQWFERRKNDPVGTARAA
KLSMKTHVEAMLAFHAQGIPTFDYGNNIRQMAQDVGCDNAFAFPGFVPAYVRPLFCRGIG
PFRWVALSGDPEDIYKTDQKVKELIPDDAHLHHWLDMAKERISFQGLPARICWVGLGLRD
KLGLAFNEMVRNGELKAPVVIGRDHLDSGSVASPNRETEAMKDGSDAVSDWPLLNAMLNV
AGGATWVSLHHGGGVGMGYSQHSGIVIVCDGTAEADKRIARVLWNDPGTGVMRHADAGYE
IAVECAKEQGLKLPMV