Protein Info for LRK55_RS15795 in Rhodanobacter denitrificans MT42

Annotation: homoserine O-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01392: homoserine O-acetyltransferase
  • amino acids 13 to 363 (351 residues), 360.4 bits, E=4.8e-112
• PF00561: Abhydrolase_1
  • amino acids 42 to 350 (309 residues), 67.2 bits, E=9.1e-23

Best Hits

Swiss-Prot: 79% identical to SST_FRAAD: Serine O-succinyltransferase (Fraau_1049) from Frateuria aurantia (strain ATCC 33424 / DSM 6220 / NBRC 3245 / NCIMB 13370)

KEGG orthology group: K00641, homoserine O-acetyltransferase [EC: 2.3.1.31] (inferred from 66% identity to sml:Smlt3277)

MetaCyc: 60% identical to serine O-acetyltransferase (Streptomyces lavendulae lavendulae)
Serine O-acetyltransferase. [EC: 2.3.1.30]

Predicted SEED Role

"Homoserine O-acetyltransferase (EC 2.3.1.31)" in subsystem Methionine Biosynthesis (EC 2.3.1.31)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (8/9 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-cysteine biosynthesis I (2/2 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• L-homocysteine biosynthesis (1/2 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• L-cysteine biosynthesis VII (from S-sulfo-L-cysteine) (2/4 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (4/7 steps found)
• N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis (1/4 steps found)
• D-cycloserine biosynthesis (2/6 steps found)
• superpathway of seleno-compound metabolism (6/19 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Methionine metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.31

Use Curated BLAST to search for 2.3.1.30 or 2.3.1.31

Protein Sequence (369 amino acids)

>LRK55_RS15795 homoserine O-acetyltransferase (Rhodanobacter denitrificans MT42)
MGDARRYFALPSPFPMKRGGQLHGAHVAYETWGTLNAARDNAVLVLTGLSPSAHMTSCEE
DPSPGWWQEMAGPGKAIDTTRWFVICVNSLGSDKGSTCPASIDPATGEAYRLSFPELALE
DVASAAHAVVGSLGITRLACLIGCSMGGMSALAYMLLHPGSVRTHISVDTAPQAQPFAIA
IRSLQREAIRLDRHWNEGVYDDDNYPDIGMSIARKLGVITYRSAMEWNGRFARIRLDPEQ
REDEPFGREFQVESYLEGHARRFVRHFDPNSYLYLSRASDWFDIAEYGHGSVLEGLKRIH
IEQAMVIGVSTDILFPLQQQEQIAEGLEAAGARVEFVALDSPQGHDAFLVDIDNYSRAIG
GFLNRLPAS