Protein Info for LRK55_RS14820 in Rhodanobacter denitrificans MT42

Annotation: polynucleotide adenylyltransferase PcnB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 TIGR01942: poly(A) polymerase" amino acids 15 to 408 (394 residues), 491.7 bits, see alignment E=8.7e-152 PF01743: PolyA_pol" amino acids 46 to 174 (129 residues), 126.5 bits, see alignment E=1.3e-40 PF12627: PolyA_pol_RNAbd" amino acids 201 to 263 (63 residues), 58.7 bits, see alignment E=6e-20 PF12626: PolyA_pol_arg_C" amino acids 317 to 441 (125 residues), 121.3 bits, see alignment E=3.4e-39

Best Hits

Swiss-Prot: 54% identical to PCNB_SALTY: Poly(A) polymerase I (pcnB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 62% identity to xcv:XCV1814)

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (450 amino acids)

>LRK55_RS14820 polynucleotide adenylyltransferase PcnB (Rhodanobacter denitrificans MT42)
MNLESRTDATPTLRVIPRDQHIISRKNISKSALRVLYRLNEAGYAAYLVGGAVRDLLLGL
RPKDFDVATNATPDEVKKLFRNCRLIGRRFRLAHVVFGPEIIEVATFRGTGEEGGEGDRH
IIDGRIVRDNIWGTIEEDAVRRDFRVNAMYYDISDFSVRDYVGGMRDLEDRMLRLIGDPD
TRYHEDPVRMLRAARLAAKLGMHIDAAAMEPFETLGPLLADAAPARLFDESLKMFLSGHG
LKSFRVLEQCGLLKFLFPATARALKRGDEALRALVEHGLANTDARIAEGKSVTPAFLFAV
LLWGEVRDVAHQLIGRGVEGNEAWSRAAAQVVGEQCQRVAIPRRFTFTMEEIWSLQPRFE
QVQRKRVFRLMAHPRFRAAFDFLLLRGDESPAMRELGQWWAHAQQLPPDVLAAALPAGGG
SDHVVAAPVAARPPRARRRRRRPADKSGSA