Protein Info for LRK55_RS13235 in Rhodanobacter denitrificans MT42

Annotation: flagellar basal-body MS-ring/collar protein FliF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 transmembrane" amino acids 31 to 50 (20 residues), see Phobius details amino acids 470 to 491 (22 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 26 to 565 (540 residues), 313.7 bits, see alignment E=1.6e-97 PF01514: YscJ_FliF" amino acids 52 to 227 (176 residues), 191.5 bits, see alignment E=1.2e-60 PF08345: YscJ_FliF_C" amino acids 259 to 446 (188 residues), 154.6 bits, see alignment E=2.7e-49

Best Hits

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (567 amino acids)

>LRK55_RS13235 flagellar basal-body MS-ring/collar protein FliF (Rhodanobacter denitrificans MT42)
MADNAIATTANNGTRLDPFKQLARNPATRQLVLLVAVAAAVALGVAVVLWSRGPNYGLLY
AGLEQKDASAITQELQAGNTPYQLSSDGSSIMAPAADLAALRLKLAAKGLPQGSASSSAL
PAADSPFGMSDLAERTRYQQLLEADLGATIGGLQSVRAARVHLALPKPSAFIRDSREASA
SVLVTLYPGRQLDAGQVAAIVHLVAASVPNLEARQVSVIDQQGQLLTTDPESPGAVGDNR
LRIATRIENTYAQRIEELLMPLVGPGRVHAQVHADLDFSRTEKATETFGHDHPALRSEQT
SSEQRGADAAGGGVPGALSNQPPVTVAQPTAAKPDAGKAAAATATAATTNPGESSSSATR
NYELDRTISHVSDPAGRLARLTVAVVVDDKLAVPANPDASADDATAAPKSVPFSAQELQH
LTELTKNAVGFDAARGDSVSVVNQAFQRGAAPEALPETPLWGRPGVLDLLKQGAGVLIAL
LVAFGLLRPLLKGLLRGEPAARALPSPMPQISVRVDDELAANEPARLGGAMPTLSYEQRV
GQARRMVGENSKQVAQVVRNWVSEDGN