Protein Info for LRK55_RS12365 in Rhodanobacter denitrificans MT42

Annotation: bifunctional aspartate kinase/diaminopimelate decarboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 1 to 278 (278 residues), 144.2 bits, E=8.8e-46
• TIGR00657: aspartate kinase
  • amino acids 1 to 447 (447 residues), 298.1 bits, E=1.3e-92
• TIGR01048: diaminopimelate decarboxylase
  • amino acids 496 to 852 (357 residues), 313.6 bits, E=1.6e-97
• PF02784: Orn_Arg_deC_N
  • amino acids 508 to 741 (234 residues), 126.5 bits, E=1.7e-40
• PF00278: Orn_DAP_Arg_deC
  • amino acids 711 to 831 (121 residues), 86.9 bits, E=1.3e-28

Best Hits

KEGG orthology group: K12526, bifunctional diaminopimelate decarboxylase / aspartate kinase [EC: 2.7.2.4 4.1.1.20] (inferred from 56% identity to xom:XOO_1317)

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Diaminopimelate decarboxylase (EC 4.1.1.20)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis (EC 2.7.2.4, EC 4.1.1.20)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• spermidine biosynthesis II (2/4 steps found)
• ectoine biosynthesis (2/5 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• norspermidine biosynthesis (2/6 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• superpathway of polyamine biosynthesis III (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.7.2.4, 4.1.1.20

Use Curated BLAST to search for 2.7.2.4 or 4.1.1.20

Protein Sequence (853 amino acids)

>LRK55_RS12365 bifunctional aspartate kinase/diaminopimelate decarboxylase (Rhodanobacter denitrificans MT42)
MKFGGTSVATLPRWQNIRELVASRRAEGARVLVVVSALTGITDALKQLCGEGGKDKRKAA
AGAVAQRHYDLLEHMQLALPDTLGSRLSDLAALAADGPAPRGELAWQAQVQAHGELMSSA
LGAAFLSHSGLPTQWLDARECLAAVALPNQNERTRLLSAMVEAKPDPALNARLAALGEVF
ITQGFIAREQHGRTVLLGRGGSDTSAAYFGALLKAQRVEIWTDVAGMFTANPRQVPGARL
LQRLDYEEAQEIASTGAKVLHPRCLSPLREPRVPLLIKDTNRPELDGTVIGPEVRAHAPS
VKAISARKGITLVSMESVGMWQQVGFLADVFAHFKTHGLSVDLIGSAETNVTVSLDPTEN
LLDSDAIAALASDLAKVCRVKVIAPCAAITLVGRGMRSLLHTLSGVLAEFGQLRVHLISQ
SSNNLNLTFVVDEEVVDALLPHLHDLLIGAGALRTDDSVLFGPSWQMLYGGGDIVPAVPA
WWRVAQRPRLLELAAAATPRYVYHLPTVRQQAHELKSLAAVDRLHYAVKANTHPAILRTL
AAEGFAFECVSPGELEAVSAVVPASVPLLFTPNFAPRRDYVQALATRASVTLDALHPLQH
WGELFRGREIVLRVDLGRGLGHHEKVRTGGSASKFGLPLDQLPTFLQLADAHGVAVRGLH
AHLGSGVLDAAHWGEVYAQLASLAERIASVVFLNIGGGLGVPAHPGEASLDIAALDRALR
EVKAAYPQYQLWMEPGRYLVADAGVLLTRVTQLKDKGGFRYLGVDTGMNSLIRPALYDAW
HKIVNLSRLGEPATALYQVVGPICESGDVLGTDRRLPEAAEGDVILIAQAGAYGKVMSSH
YNLRDEADEVIID