Protein Info for LRK55_RS12160 in Rhodanobacter denitrificans MT42

Annotation: 1-deoxy-D-xylulose-5-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13292: DXP_synthase_N
  • amino acids 11 to 281 (271 residues), 391.1 bits, E=7.7e-121
• TIGR00204: 1-deoxy-D-xylulose-5-phosphate synthase
  • amino acids 12 to 617 (606 residues), 872 bits, E=1.2e-266
• PF02775: TPP_enzyme_C
  • amino acids 113 to 182 (70 residues), 23.2 bits, E=1.6e-08
• PF00676: E1_dh
  • amino acids 119 to 188 (70 residues), 24.1 bits, E=5e-09
• PF02779: Transket_pyr
  • amino acids 318 to 478 (161 residues), 148.7 bits, E=4.2e-47
• PF02780: Transketolase_C
  • amino acids 494 to 610 (117 residues), 85.6 bits, E=8.2e-28

Best Hits

Swiss-Prot: 70% identical to DXS_XANC5: 1-deoxy-D-xylulose-5-phosphate synthase (dxs) from Xanthomonas campestris pv. vesicatoria (strain 85-10)

KEGG orthology group: K01662, 1-deoxy-D-xylulose-5-phosphate synthase [EC: 2.2.1.7] (inferred from 71% identity to psu:Psesu_1183)

Predicted SEED Role

"1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)" in subsystem Isoprenoid Biosynthesis or Pyridoxin (Vitamin B6) Biosynthesis or Thiamin biosynthesis (EC 2.2.1.7)

MetaCyc Pathways

• methylerythritol phosphate pathway II (9/9 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
• taxadiene biosynthesis (engineered) (11/13 steps found)
• methylerythritol phosphate pathway I (8/9 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (10/12 steps found)
• pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
• isoprene biosynthesis I (8/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• superpathway of thiamine diphosphate biosynthesis II (7/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (3/6 steps found)
• superpathway of thiamine diphosphate biosynthesis I (5/10 steps found)
• superpathway of ergosterol biosynthesis II (10/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.2.1.7

Protein Sequence (629 amino acids)

>LRK55_RS12160 1-deoxy-D-xylulose-5-phosphate synthase (Rhodanobacter denitrificans MT42)
MNDLSHYPHLAPIETPADLRRVSDDDLPAVADELRQYLIESVASSGGHFGAGLGVVELTV
ALHHEFDTPHDRLVWDVGHQCYPHKILTGRRDRITTIKKKDGLAPFPRREESAYDTFGVG
HSSTSISAALGMAIAAQRKGDKRKVVAVIGDGAMTAGMAFEALNHGGDVEPDMLVVFNDN
GMSISENVGAMTKMMARAMASRRLNQLRERAKRAIPKQSFAGRWFKRWEEHAKGMFVPST
LFEELGFHYTGPIDGHNIPQLLQALRTVKNLPGPQLLHVITTKGKGYAPAEQAQIEYHAV
GPFDPEAGVVKKPAPARPTYTDIFSDWLCDQAAADERLLGITPAMREGSGLVRFSKEYPE
RYFDVAIAEQHAVTLAAGMACEGAKPVVAIYSTFLQRAYDQAIHDVALQNLDVTFAIDRA
GVVGPDGATHSGSFDLTYLRCLPHMVIMAPADENECRMMLSTGFHHHGPAAIRYPRGTGP
GVAIHQELDTLPIGKAELRRRGHGLALLSFGAMLAPAAAIAAEFDATLVNMRFVKPLDEA
LLVELAKTHDGFVTLEDNAVAGGAGSAVAECLAAHGIVLPILHLGLPDVYLEHGSREEVL
SMAGLDLPGIRHAIRARFPQAGTLSQASA