Protein Info for LRK55_RS11465 in Rhodanobacter denitrificans MT42
Annotation: RdgB/HAM1 family non-canonical purine NTP pyrophosphatase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 62% identical to IXTPA_XANOR: dITP/XTP pyrophosphatase (XOO1141) from Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
KEGG orthology group: K01516, nucleoside-triphosphatase [EC: 3.6.1.15] (inferred from 63% identity to xal:XALc_0794)MetaCyc: 52% identical to dITP/XTP pyrophosphatase (Escherichia coli K-12 substr. MG1655)
Nucleotide diphosphatase. [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]; 3.6.1.66 [EC: 3.6.1.66, 3.6.1.9]
Predicted SEED Role
"Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15)" in subsystem Heat shock dnaK gene cluster extended (EC 3.6.1.15)
MetaCyc Pathways
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
- dZTP biosynthesis (4/5 steps found)
- pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
- UTP and CTP dephosphorylation I (5/7 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
- pyrimidine deoxyribonucleotides dephosphorylation (2/3 steps found)
- tunicamycin biosynthesis (2/9 steps found)
KEGG Metabolic Maps
- Nicotinate and nicotinamide metabolism
- Pantothenate and CoA biosynthesis
- Purine metabolism
- Riboflavin metabolism
- Starch and sucrose metabolism
- Thiamine metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.6.1.9
Use Curated BLAST to search for 3.6.1.15 or 3.6.1.66 or 3.6.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (199 amino acids)
>LRK55_RS11465 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase (Rhodanobacter denitrificans MT42) MSRIVLASSNPGKLKEFGALLAGSGLDVIPQASLGIRDADETGHTFIENALLKARHAAQA SGLPALADDSGLCVDHLHGAPGLYSARYSGGHGDATANNAKLLRELDGVTVERRGAFFIC VLVLLQHAGDPAPLIAEARWHGRILTEGRGSGGFGYNPVFLPDGYEQSVAEMDDALKNRL SHRGQALAKLHDRLRELRP