Protein Info for LRK55_RS10600 in Rhodanobacter denitrificans MT42

Annotation: dihydroorotase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00856: dihydroorotase, homodimeric type
  • amino acids 6 to 344 (339 residues), 449 bits, E=4.7e-139
• PF01979: Amidohydro_1
  • amino acids 13 to 320 (308 residues), 68.8 bits, E=2.6e-23

Best Hits

Swiss-Prot: 66% identical to PYRC_METFK: Dihydroorotase (pyrC) from Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

KEGG orthology group: K01465, dihydroorotase [EC: 3.5.2.3] (inferred from 67% identity to afr:AFE_1672)

MetaCyc: 53% identical to dihydroorotase (Escherichia coli K-12 substr. MG1655)
Dihydroorotase. [EC: 3.5.2.3]

Predicted SEED Role

"Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis (EC 3.5.2.3)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.3

Use Curated BLAST to search for 3.5.2.3

Protein Sequence (350 amino acids)

>LRK55_RS10600 dihydroorotase (Rhodanobacter denitrificans MT42)
MTPTRLEITRPDDWHLHLRDGDALASVIGHTAKRFARAIVMPNLKPPVTTVAQAEDYRGR
ILASLPAGSRFQPLMTLYLTEDTPVDEIARAKDSGSVFAVKYYPAGATTNSQSGVRELSR
VYAVIEAMEKHELPLLMHGEVTDPAIDIFDRERVFIERHLLPLRERFPALRMVLEHITTR
DAAEFVAGAPANVAATITAHHLLLNRNALFEGGIRPHNYCAPILKRETHRAALLQAATSG
DAHFFLGTDSAPHPREAKETACGCAGLYTAHAALELYAEAFEQVGRLDRLEAFASFRGAD
FYHLPRNQERIALERTPWIVPEEYPLAASTCRPMRAGEAVAWSVVEAAPR