Protein Info for LRK55_RS10415 in Rhodanobacter denitrificans MT42

Annotation: glycerol-3-phosphate 1-O-acyltransferase PlsB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 892 transmembrane" amino acids 623 to 640 (18 residues), see Phobius details TIGR03703: glycerol-3-phosphate O-acyltransferase" amino acids 22 to 819 (798 residues), 946.2 bits, see alignment E=9.3e-289 PF01553: Acyltransferase" amino acids 305 to 434 (130 residues), 74.8 bits, see alignment E=6.1e-25 PF19277: GPAT_C" amino acids 445 to 789 (345 residues), 360.7 bits, see alignment E=9.4e-112

Best Hits

Swiss-Prot: 61% identical to PLSB_XANAC: Glycerol-3-phosphate acyltransferase (plsB) from Xanthomonas axonopodis pv. citri (strain 306)

KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 60% identity to psu:Psesu_2936)

Predicted SEED Role

"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (892 amino acids)

>LRK55_RS10415 glycerol-3-phosphate 1-O-acyltransferase PlsB (Rhodanobacter denitrificans MT42)
MTTTADSSARPRAPWWFNLAGQLLQPWVRIRRDPAEPAALLQAGVPVCYVIERDGFSDAL
ILQRACREAGLPNPMRPLAGTRRRRSVFALARRDGSLLGRNRKRNPNEALRQLVHSLEGL
PDSDIQIVPVSIYVGRAPSRDSGWFRVLFSENWVMVGRFRRMLALLLNGRDTVVHFSTPV
SLRQTLDESGGITPERFARKVARVLRTHFHRIRAAVIGPDLSHKRTVVDAVLNAEPVRAA
IAAAAAKEKISHAKAWRKAHQLVLEIAADYSHPVVRSASFLLSNFWNKLYDGIAMHHFDK
ARAAAPGHEVIYVPCHRSHADYLLMSYQLHVSGVVVPHIAAGVNLNLPVIGPILRRGGAF
FLRRSFRGNALYSVVFNEYVAQLIDRGVPMEYFIEGGRSRTGRLLAPRAGMLAMTVRAFL
RAPRRPVLFQPVYIGYEKLMEGKSYIGELSGQPKEKESLLGLLKGLKVLRQRYGHVALNF
GEPIELTPLLDAASHDWRAAGADPEAKPEWLGAVTDRLAEQIQVNINRAADVNPINLLAL
ALLATPKHAMAESDLLTQLELGKTMLEELPYSDRITLTPMNPAAIIAYGEQMGWIQRVQH
PLGDVLTATGERAVLLSYFRNNVLHLTATAAWVACCFLNNRRMSRASILRLGRIIYPFIQ
GELFLPWDTDGFAEQLQATIDFFVRRGLLEAVSDGRVLERSPGQDDGAYQLKVIARSLIQ
AFERYYITIAALAKNGPHTMTGAELENACSLTAQRLSLLNELTAPEFFDKALFRGFIQKL
RERRIVWTDDAGKLDYDAALEGMVRDARVILSREVRHSILKITPGGNDWEAPAADAREGA
DQGTYVPADSDDAALHARHVAAEQREHERRSGSDRRLPDAAEPTPTHRDAAE